OneScript® Plus cDNA Synthesis Kit

OneScript® Plus cDNA Synthesis Kit

Cat# Unit
G236100 rxn

Documents

Category:

Description

Description

OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C – 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

 

Key Features

  • Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
  • Robust cDNA synthesis from any RNA template
  • High reproducibility and excellent yield
  • Employs abm‘s OneScript® Plus Reverse Transcriptase (Cat. No. G237)
  • Earn 2X the rewards points

 

PRODUCT COMPONENT QUANTITY
OneScript® Plus Reverse Transcriptase 100 rxn (100 µl)
5X RT Buffer 400 µl
Oligo(dT) (10 μM) 100 µl
Random Primers (10 μM) 100 µl
dNTP (10 mM) 100 µl
Nuclease-Free H2O 2 x 1.0 ml

 

 

SKU G236
Applications
  • Synthesizing cDNA from ssRNA
  • DNA primer extension
  • Sequencing dsDNA
  • Constructing cDNA library
  • Producing template for use in RT-PCR
  • Labelling 3′-end of duplex DNA via end-filling reactions
  • Generating probes for hybridization
Storage Condition Store at -20°C.
Unit Quantity 100 rxn

References

  • Menon, V et al. “The contribution of visceral fat to improved insulin signaling in Ames dwarf mice” Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
  • Lee, G et al. “Potential monoclonal antibody therapy for the treatment of ovarian cancer” Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Menon, V et al. “The contribution of visceral fat to improved insulin signaling in Ames dwarf mice” Aging Cell. 13(3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
  • Juskeviciute, E et al. “Inhibition of miR-21 rescues liver regeneration after partial hepatectomy in ethanol-fed rats” Amer. J. Physiol.-Gastrointestinal and Livery Physiology 5:G794-G806 (0). DOI: 10.1152/ajpgi.00292.2016 .
  • Amodio, G et al. “Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach” Cell Bio. Toxicol. 285: (2016). DOI: 10.1007/s10565-016-9335-z.
  • Antony, A et al. “MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration” Nat. Comm. : (2016). DOI: 10.1038/ncomms10955.
  • Lakshmi , K et al. “A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration” BMC Genomics 1:1 (2016). DOI: 10.1186/s12864-016-2492-x.
  • Maher, SK et al. “Rethinking the biological relationships of the thyroid hormones, l-thyroxine and 3,5,3′-triiodothyronine” Comp. Biochem. Physiol. D: Genomics Proteomics :44-53 (2016). DOI: http://dx.doi.org/10.1016/j.cbd.2016.04.002.
  • Diana, D et al. “Inhibition of autocrine signaling of the Epithelial-Mesenchymal Transition in breast cancer” : (2016).
  • Park, H-S et al. “PPARγ neddylation essential for adipogenesis is a potential target for treating obesity” Cell Death Diff. :45-57 (2016). DOI: http://dx.doi.org/10.1016/j.bcp.2016.03.018.
  • Gao, J., Song, J., Du, M., & Mao, X. “Bovine α-lactalbumin hydrolysates (α-LAH) attenuate high-fat diet induced nonalcoholic fatty liver disease by modulating hepatic lipid metabolism in C57BL/6J mice” Journal of Functional Foods 54:254–262 (2019). DOI: 10.1016/j.jff.2019.01.027.
  • Saliba, J., Coutaud, B., Solovieva, V., Lu, F., & Blank, V. “Regulation of CXCL 1 chemokine and CSF 3 cytokine levels in myometrial cells by the MAFF transcription factor” Journal of Cellular and Molecular Medicine 23(4):2517–2525 (2019). DOI: 10.1111/jcmm.14136.
  • ŞEN, A., Ayar, B., Yilmaz, A., ACAR, Ö. Ö., Turgut, G. C., & TOPÇU, G. “Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption”  Turkish Journal of Chemistry  43(5):1350-1364 (2019).
  • Sun, L., Pang, Y., Wang, X., Wu, Q., Liu, H., Liu, B., … Jiang, C. “Ablation of gut microbiota alleviates obesity-induced hepatic steatosis and glucose intolerance by modulating bile acid metabolism in hamsters” Acta Pharmaceutica Sinica B 9(4):702–710 (2019). DOI: 10.1016/j.apsb.2019.02.004.
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. “PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε” Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. “Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress” Metallomics. : (2019).

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