BlasTaq™ HotStart DNA Polymerase
Cat# | Unit |
---|---|
G595 | 400 rxn |
Documents
Description
Description
Eliminate non-specific amplification and primer-dimer formation!
BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.
During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.
Product Features:
- No non-specific amplification or primer-dimers
- Improves yield of desired product
- Simplifies reaction setup
- Earn 2X the rewards points
PRODUCT COMPONENT | QUANTITY |
---|---|
BlasTaq™ HotStart DNA Polymerase | 400 rxn (200 µl) |
5X BlasTaq™ Buffer 1 | 2 x 1.0 ml |
SKU | G595 |
---|---|
Storage Condition |
Store at -20°C. |
Unit quantity | 400 rxn |
Supporting Protocol
MSDS
QC
Other
- Chung, G et al. “CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans” DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.