Overview
Introducing Twist Alliance Panels, a curated collection of high-quality target enrichment panels designed in partnership with leading scientific institutions.
In combining Twist’s world-class oligonucleotide synthesis platform with our scientific partners’ deep area expertise, Twist Alliance panels provide ready-made panels to help accelerate your research. Why spend time designing panels when experts have already done the work for you?
Explore Our Panels
The Twist Alliance Panels are designed to give you a head start, bringing together expertise from multiple disciplines for superior target enrichment, for applications ranging from cancer diagnostics to carrier screening. If none of the panels look quite right for your needs, each panel can be customized with spike-in coverage where you need it. Are you interested in partnering with us to commercialize a new panel?
Helping you skip the design, build, test, learn cycle.
ALLIANCE PANELS
Twist Alliance Diversity SNP Panel
A prevalent issue with historical genotyping tools is their bias toward a small number of ethnicities, leaving significant portions of humanity underrepresented in classic genotyping workflows. As an example, microarray platforms only offer fixed content updated occasionally, compounding this challenge.
Researchers can solve these inefficiencies and inequities by utilizing target capture-based NGS workflows with probe panels designed for diversity and flexibility.
As part of Twist’s growing Targeted GBS portfolio, the Twist Diversity SNP Panel gives researchers a new flexible ethnicity-neutral gold standard to use for GBS.
Scientists have the freedom to use the panel as a stand-alone genotyping panel or as a spike-in into the Twist Human Comprehensive Exome panel. Its design is also customizable—additional probes covering regions of interest can be synthesized on Twist’s DNA synthesis platform.
The Twist Diversity SNP Panel content was curated in partnership with leaders at the Regeneron Genetics Center. Designed to integrate into existing fully automated exome processing workflows, this panel provides base calls and imputed variants.
Genotype imputations can be calculated using open source tools available. Please reach out to Customer Support to get a copy of our imputation guide to getting started with imputation analysis tools.
Note: The Diversity SNP panel content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.
PROBES: 640k
SNPs COVERED: 1.4M
SENSITIVITY: Imputation of most variants down to MAF of 0.1% or less
Twist Alliance Pan-cancer Methylation Panel – 1.5 MB
Methylation sequencing is a sensitive tool for detecting and tracing the origin of cancer cells. But like any sequencing project, there is a tradeoff between breadth and depth of coverage. If you’re ready to focus on cancer-specific methylation patterns, this is the panel for you. Experts at AnchorDx partnered with Twist to create a custom target enrichment panel that focuses specifically on targets relevant to 31 different cancers.
The Twist Alliance Pan-cancer Methylation Panel – 1.5 MB provides deep coverage of clinically focused targets that allow for the study of methylation patterns that may be relevant to early cancer detection and diagnosis from tumor and liquid biopsy samples. Designed in collaboration with experts at AnchorDx and constructed using Twist’s world-class oligonucleotide synthesis platform, this panel provides a high on-target rate and high uniformity, and has been validated with the Twist Methylation workflow using liquid biopsy samples of breast cancer, colorectal cancer, and non-small cell lung cancer.
Panel Design
- 31 cancer types, 47 disease entities, based on TCGA database
- 13,090 Probes
- 126k CpG sites involved
- ~12k Differentially Methylated Regions (DMRs)
- 9k of 12k (74%) overlapped with the CpG islands
Note: The Twist Alliance Pan-cancer Methylation Panel – 1.5 MB is not ISO-13485 certified
Twist Alliance Clinical Research Exome – 34.9 MB
The Twist Alliance Clinical Research Exome helps support the Broad Institute Genomics Platform and was designed using validated data from clinical patient samples. The panel leverages Twist’s flexible NGS platform to customize content easily and quickly, resulting in a comprehensive survey of the exome with supplemental enrichment of clinically relevant areas of the genome related to cancer as well as rare and inherited diseases. By leveraging the best-in-class uniformity of Twist NGS probes, the assay enables a per sample cost and throughput efficiency that Broad has already leveraged to process more than 250,000 samples to date, keeping it on the leading edge of exome sequencing.
The full design of this panel includes the Twist Core exome, the mitochondrial genome, and additional validated coding and non-exonic regions of interest such as the ACMG73 genes, supplemental coverage of regions from OMIM and COSMIC, and specific Broad-defined targets.
Note: The Twist Alliance Clinical Research Exome – 34.9 MB content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.
Note: The Twist Alliance Clinical Research Exome – 34.9 MB is not ISO-13485 certified.
Resources
Twist Alliance VCGS Exome – 40.1 MB
Where routine testing has failed to provide a diagnosis, physicians often turn to DNA sequencing panels for answers, particularly when a heritable disease is suspected. But without expert guidance, it can be hard to know which panel is the right panel.
Drawing on decades of experience in clinical genomics, Victorian Clinical Genetics Services (VCGS) has partnered with Twist to design a diagnostic panel—Twist Alliance VCGS Exome – 40.1 MB—that covers the whole exome, with dedicated coverage boosting over clinically relevant genes (Mendeliome). In designing this panel, special care was given to ensuring additional coverage of clinically relevant loci that fall outside the gene coding regions traditionally targeted by exomes (i.e. known pathogenic loci in non-coding regions).
The Twist Alliance VCGS Exome – 40.1 MB panel is a best-in-class, highly uniform panel that can help streamline the identification of heritable disease-linked alleles, be it through carrier screening or pre- and post-natal testing.
Note: The Twist Alliance VCGS Exome – 40.1 MB content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.
Note: The Twist Alliance VCGS Exome – 40.1 MB is not ISO-13485 certified.
Total probes: 425.4K
Includes the Twist Comprehensive Exome of: 394.1K probes
and content curated by VCGS of: 31.3K probes
Ordering Information
104282: Twist Alliance VCGS Exome – 40.1 MB, 2 Reactions Kit
104283: Twist Alliance VCGS Exome – 40.1 MB, 12 Reactions Kit
104284: Twist Alliance VCGS Exome – 40.1 MB, 96 Reactions Kit
Twist Alliance Canine Exome – 40.5 Mb
Where routine testing has failed to provide a diagnosis, physicians often turn to DNA sequencing panels for answers, particularly when a heritable disease is suspected. But without expert guidance, it can be hard to know which panel is the right panel.
Drawing on decades of experience in clinical genomics, Victorian Clinical Genetics Services (VCGS) has partnered with Twist to design a diagnostic panel—Twist Alliance VCGS Exome – 40.1 MB—that covers the whole exome, with dedicated coverage boosting over clinically relevant genes (Mendeliome). In designing this panel, special care was given to ensuring additional coverage of clinically relevant loci that fall outside the gene coding regions traditionally targeted by exomes (i.e. known pathogenic loci in non-coding regions).
The Twist Alliance VCGS Exome – 40.1 MB panel is a best-in-class, highly uniform panel that can help streamline the identification of heritable disease-linked alleles, be it through carrier screening or pre- and post-natal testing.
Note: The Twist Alliance VCGS Exome – 40.1 MB content has not been fully validated through the complete Twist workflow. For recommendations on assay set-up, please contact Twist Customer Support.
Note: The Twist Alliance VCGS Exome – 40.1 MB is not ISO-13485 certified.
Total probes: 425.4K
Includes the Twist Comprehensive Exome of: 394.1K probes
and content curated by VCGS of: 31.3K probes
Ordering Information
104282: Twist Alliance VCGS Exome – 40.1 MB, 2 Reactions Kit
104283: Twist Alliance VCGS Exome – 40.1 MB, 12 Reactions Kit
104284: Twist Alliance VCGS Exome – 40.1 MB, 96 Reactions Kit
