Fast Hybridization and Wash Kit

Balance Workflow and Performance Needs with Maximum Confidence As demonstrated with the Twist Human Core Exome Kit:

The 15-minute hybridization yields the same excellent performance (depth of coverage) previously achieved with overnight hybridization
Longer 1–4 hour hybridizations in the Twist Fast Hybridization Solution improve performance
Can also be used in workflows that require an overnight hybridization *
This allows you to adjust the hybridization time as needed to balance your workflow and performance needs.

*Our standard hybridization solution is also still available as a component of the Twist Hybridization and Wash Kit (101025 or 101026).

Rapid, Streamlined, and Automatable Workflow

Twist Fast Hybridization Solution is a component of a streamlined target enrichment workflow that can be completed in as little as 5 hours.

Consistent Performance From Run to Run

Repeatable performance ensures confidence in results. When experimental conditions are held constant, the Twist Library Preparation EF Kit 2.0 produces a robust, consistent DNA library fragment size. Shown here are 8 different electropherograms of NGS libraries generated with the kit. The overlap of the fluorescent curves demonstrates the consistency in library preparation that can be achieved from run to run.

NGS libraries were generated with the Twist Library Preparation EF Kit 2.0 and Twist’s Universal Adapter system. 50 ng of high-quality gDNA was fragmented for 20 minutes at 37°C. 6 cycles of PCR were utilized for amplification. Samples were analyzed with an Agilent DNA 7500 assay and results overlaid in Expert 2100 software.

Tune fragment size to fit your experimental needs

With the Twist Library Preparation EF Kit 2.0, DNA library fragment size can be tuned to your specific needs by simply adjusting fragmentation time. Shown here are four electropherograms of NGS libraries generated using differing fragmentation times.

NGS libraries were generated with the Twist Library Preparation EF Kit 2.0 and Twist’s Universal Adapter system. 50 ng of high-quality gDNA was fragmented for various times at 37°C. 6 cycles of PCR were utilized for amplification. Samples were analyzed with an Agilent DNA 7500 assay and results overlaid in Expert 2100 software.

Improved library amplification to minimize sequencing error

Errors during amplification are unavoidable, but they need not be common. The upgraded Twist Library Preparation EF Kit 2.0 includes the Equinox Master Mix, featuring a high-fidelity hot-start enzyme. Compared to a previously recommended and commonly used polymerase, the Equinox Master Mix demonstrates a lower error rate with fewer misincorporated bases. This means more accurate amplification and ultimately sequencing data you can trust.

Base misincorporation events across numerous pairs of mismatched bases were measured with a proprietary NGS-based assay. Values presented represent an average error rate of all misincorporation events.