Oligo Clean & Concentrator
| Cat# | Name | Size |
|---|---|---|
| D4060 | Oligo Clean & Concentrator | 50 Preps |
| D4061 | Oligo Clean & Concentrator | 200 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS): D4060
SDS (MSDS): D4061
Description
Highlights
- Fast & Simple: Clean and concentrate DNA and RNA oligonucleotides in 2 minutes.
- Ultra-Pure: Ready for hybridization, Next-Gen Sequencing, PCR, ligations, etc.
- Complete removal: Eliminate dyes, salts, enzymes, nucleotides, and short oligos.
Description
The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.
Applicable For
Single-stranded and double-stranded DNA and RNA
Elution Volume
≥ 6 µl
Equipment
Microcentrifuge
Purity
A260/A280 > 1.8, A260/A230 > 1.8
Sample Source
Enzymatic reaction mixtures containing oligonucleotides ≥ 16 nt (radioactive-, biotin-, DIG-labeled, etc.)
Size Range
For oligonucleotides ≥ 16 nt, up to 23 kb
Yield
10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery > 90%
 |
99 / 100 Bioz Stars |
Repurposing CRISPR-Cas12b for mammalian genome engineering
Cell Discov – Published 27 Nov 2018
Transcribed RNAs were purified using Oligo Clean & ConcentratorTM (ZYMO Research) and quantitated on NanoDropTM 2000 (Thermo Fisher) were in vitro-transcribed using HiSribeTM T7 Quick High Yield RNA Synthesis Kit (NEB) and PCR-amplified DNA templates carrying a T7 promoter sequence. . Transcribed RNAs were purified using Oligo Clean & ConcentratorTM (ZYMO Research) and quantitated on NanoDropTM 2000 (Thermo Fisher).. Primers used for template preparation are listed in Supplementary Table .
Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow me…
Nat Commun -Published 14 Nov 2018
he mRNA fragments were purified with Oligo Clean & ConcentratorTM (Zymoresearch D4061) and then subjected to immunoprecipitation with α-m6 A antibody (1:100, Synaptic Systems, Cat No: 202003).
Regulatory Targets of the Response Regulator RR_1586 from Clostridioide…
J Bacteriol – Published 6 Nov 2018
EmeraldAmp GT master mix (Clontech) was used for all PCRs unless explicitly stated.. ZymoPURE midiprep, DNA Clean & Concentrator 5 (DNA C & C), and Oligo Clean & Concentrator (Oligo C & C) kits were purchased from Zymo Research.. Restriction enzymes, ligases, and RecA were purchased from NEB.
| Cat # | Name | Size |
|---|---|---|
| D4060-1-10 | Oligo Binding Buffer | 10 ml |
| D4060-1-40 | Oligo Binding Buffer | 40 ml |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml |
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1001-500 | Collection Tubes | 500 Pack |
| C1001-1000 | Collection Tubes | 1000 Pack |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
| C1004-250 | Zymo-Spin IC Columns | 250 Pack |
- Catalog#: D4060 / D4061
- Package Length (in Inches): 7 / 13
- Package Width (in Inches): 5 /8
- Package Height (in Inches): 4.5 / 6.5
- Package Weight (in Pounds): 0.5 / 2.6
- Size: 50 Preps / 200 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
