Exome 2.0

Overview

For best-in-class results, use best-in-class tools

Twist Exome 2.0 is designed to detect rare and inherited diseases, as well as germline cancers. This panel’s high uniformity and low off-target rate deliver best-in-class sequencing efficiency, enabling quality data to be collected with less sequencing. With superior coverage of major genetic databases (RefSeq, CCDS, GenCode, Clinvar, ACMG73 and more) and the addition of clinically relevant non-coding pathogenic and likely pathogenic variants, Twist Exome 2.0 provides the value of multiple clinical panels all wrapped into one, easily customizable package.

Coverage That Matters
  • Covers 36.5 Mb of human protein coding regions
  • Based on recent database releases
  • Includes carefully curated clinical content
Best-in-Class Performance
  • Uniform enrichment of target regions
  • NGS QC of all probes
Retain Flexibility
  • Easily spike-in content into the exome
  • Effective across multiplex target enrichment workflows
  • Run overnight or same day workflows

Data

Best-in-Class Performance

Twist Exome 2.0 earns the title “best-in-class” by outperforming top competitors and enabling the most efficient exome sequencing. Compared to other exome panels, Twist Exome 2.0 delivers higher uniformity and a higher on-target rate as well as lower drop out and duplicate read rates. Together, this equates to fewer wasted reads and maximal coverage of target sequences.

Most complete coverage of the human exome plus more

Twist Exome 2.0 features a complete new design that maximizes coverage of clinically relevant regions from the ClinVar database while including updated coverage of protein-coding regions from CCDS, RefSeq and GENCODE. In addition, the new design also includes pharmacogenomic SNPs, extended Tert promoter coverage, and 24 Sample ID SNPs for resolving contamination issues (Pengally et al.).

Low GC bias enables target capture, regardless of GC content

Twist Exome 2.0 provides higher uniformity relative to competitors. Folded into this superior uniformity is a lower GC bias, meaning this panel can help you detect complex targets containing high, or low, levels of GC content. This means fewer rounds of sequencing are needed to adequately read targets with extreme GC content.

Most efficient exome panel

Twist Exome 2.0’s superior uniformity, on-target rate, duplicate rate, and overall coverage translates into a highly efficient, cost-saving exome panel. This efficiency enables you to sequence more samples per run, or else to achieve deeper sequencing over desired targets.

Workflow

Twist Exome 2.0 is designed to help you cover the target regions that are most important to you. To do this, we’ve built it to be amenable to a wide variety of customization options. If you’re studying calls for analysis of promoters, intronic SNPs, or UTRs, custom content can be quickly and affordably spiked into the panel to provide coverage. High-throughput studies are also enabled through sample multiplexing, up to 16-plex per reaction.

Whether you want to run the hybridization overnight or on the same day, the new Twist Standard Hyb v2 chemistry & Fast Hyb chemistry will accommodate your preference respectively.

Ordering and Specs

Product SKUs

104132: Twist Exome 2.0, 2 Reactions, Kit

104134: Twist Exome 2.0, 12 Reactions, Kit

104136: Twist Exome 2.0, 96 Reactions, Kit

105034: Twist Exome 2.0 plus Comprehensive Exome Spike-in, 2 Reactions

105035: Twist Exome 2.0 plus Comprehensive Exome Spike-in, 12 Reactions

105036: Twist Exome 2.0 plus Comprehensive Exome Spike-in, 96 Reactions