| Molecular Biology Products Pakistan

Skip to the content

info@mbps.pk
|
+92-301-8290152
|

Generic selectors

Exact matches only

Exact matches only

Search in title

Search in title

Search in content

Search in content

Search in excerpt

Search in posts

Search in posts

Search in pages

Search in pages

Hidden

info@mbps.pk
|
+92-301-8290152
|

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US

Generic selectors

Exact matches only

Exact matches only

Search in title

Search in title

Search in content

Search in content

Search in excerpt

Search in posts

Search in posts

Search in pages

Search in pages

Hidden

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US

ABM Products

Product categories

Product categories

Showing 1–16 of 18 results

100bp Opti-DNA Marker
Request A Quote
1kb Opti-DNA Marker
Request A Quote
BlasTaq™ 2X PCR MasterMix
Request A Quote
BlasTaq™ 2X qPCR MasterMix
Request A Quote
BlasTaq™ HotStart DNA Polymerase
Request A Quote
MegaFi™ One-Step RT-PCR
Request A Quote
miRNA All-In-One cDNA Synthesis Kit
Request A Quote
Mycoplasma PCR Detection Kit
Request A Quote
OneScript® Plus cDNA Synthesis Kit
Request A Quote
Safe-Green
Request A Quote
Taq DNA Polymerase
Request A Quote
OneScript Plus cDNA Synthesis Kit
Request A Quote
OneScript Plus Reverse Transcriptase (PCR)
Request A Quote
NTP Set
Request A Quote
5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
Request A Quote
dNTP Mix
Request A Quote

1
2
→

← Home → Checkout

QUICKS LINKS

Home
About Us
Privacy Policy
Terms & Conditions

BRANDS

Zymo Research
Quanta Bio
Molecular Biology
ABM
Twist Bioscience
Synthetic Viral Controls
Biolytic

Contact Details

Office no 7B-1 Fakhri Trade Center Shahrah-e – liaquat, Karachi.

+92-21-32600060

+92-301-8290152

Find Our Location

Site By: Bohradevelopers

Comparison to SYBR GreenER, ADAR

PerfeCTa SYBR Green FastMix comparison to SYBR GreenER qPCR SuperMix

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 1 pg) using PerfeCTa™ SYBR Green FastMix or the SYBR GreenER qPCR SuperMix (Invitrogen) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Replicate CT values are shown on the standard curve (Panel A, inset). Cycling conditions: Invitrogenn: 95°C, 10 min followed by 40 cycles of 95°C, 10s; 60°C, 60s; PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s. PerfeCTa SYBR Green FastMix amplified the ADAR gene with higher efficiency and greater sensitivity. All replicate reactions for SYBR GreenER qPCR SuperMix failed to amplify ADAR from 1 pg of cDNA.

Comparison to Takara, ADAR

PerfeCTa SYBR Green FastMix Comparison to SYBR PreMix Ex Taq

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa™ SYBR Green FastMix or SYBR PreMix Ex Taq™ (Takara) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. PerfeCTa™ SYBR Green FastMix produces higher fluorescent signal and detection of equal target amounts at earlier Cts. Cycling conditions for both kits: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

Comparison to Finnzymes, ADAR

PerfeCTa SYBR Green FastMix Comparison to DyNAmo Flash SYBR Green PCR Kit

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa SYBR Green FastMix or the DyNAmo Flash SYBR Green PCR Kit (Finnzymes) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Fusion of DNA-binding peptide to Tbr DNA pol results in lower specificity of the DyNAmo kit which is evident in false positive results for no template control (NTC) reactions. Chemically modified polymerase produces delayed Cts and lower signal strength compared to AccuStart™ Taq. Cycling conditions: Finnzymes: 95°C, 7 min followed by 40 cycles of 95°C, 10s; 60°C, 20s PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US