Chemicals - Molecular Biology Products Pakistan

Skip to the content

info@mbps.pk
|
+92-301-8290152
|

Generic selectors

Exact matches only

Search in title

Search in content

Post Type Selectors

info@mbps.pk
|
+92-301-8290152
|

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US

Chemicals

Product categories

Product categories

Showing 1–16 of 19 results

ZR small-RNA PAGE Recovery Kit
Request A Quote
Direct-zol Binding Buffer Concentrate
Request A Quote
RNA Extraction Buffer
Request A Quote
RNA Wash Buffer
Request A Quote
RNA Digestion Buffer
Request A Quote
RAD Buffer (RNA Agarose Dissolving Buffer)
Request A Quote
RNA Binding Buffer
Request A Quote
ZR RNA Buffer
Request A Quote
RNA Pre-Wash Buffer
Request A Quote
RBC Lysis Buffer
Request A Quote
Viral RNA Buffer
Request A Quote
Viral RNA Wash Buffer (Concentrate)
Request A Quote
RNA Extraction Buffer Plus
Request A Quote
Zymo-Seq Wash Buffer
Request A Quote
Direct-zol MagBead PreWash
Request A Quote
X-GAL (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside)
Request A Quote

1
2
→

← Home → Shop

QUICKS LINKS

Home
About Us
Privacy Policy
Terms & Conditions

BRANDS

Zymo Research
Quanta Bio
Molecular Biology
ABM
Twist Bioscience
Synthetic Viral Controls
Biolytic

Contact Details

Office no 7B-1 Fakhri Trade Center Shahrah-e – liaquat, Karachi.

+92-21-32600060

+92-301-8290152

Find Our Location

Site By: Bohradevelopers

Comparison to SYBR GreenER, ADAR

PerfeCTa SYBR Green FastMix comparison to SYBR GreenER qPCR SuperMix

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 1 pg) using PerfeCTa™ SYBR Green FastMix or the SYBR GreenER qPCR SuperMix (Invitrogen) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Replicate CT values are shown on the standard curve (Panel A, inset). Cycling conditions: Invitrogenn: 95°C, 10 min followed by 40 cycles of 95°C, 10s; 60°C, 60s; PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s. PerfeCTa SYBR Green FastMix amplified the ADAR gene with higher efficiency and greater sensitivity. All replicate reactions for SYBR GreenER qPCR SuperMix failed to amplify ADAR from 1 pg of cDNA.

Comparison to Takara, ADAR

PerfeCTa SYBR Green FastMix Comparison to SYBR PreMix Ex Taq

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa™ SYBR Green FastMix or SYBR PreMix Ex Taq™ (Takara) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. PerfeCTa™ SYBR Green FastMix produces higher fluorescent signal and detection of equal target amounts at earlier Cts. Cycling conditions for both kits: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

Comparison to Finnzymes, ADAR

PerfeCTa SYBR Green FastMix Comparison to DyNAmo Flash SYBR Green PCR Kit

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa SYBR Green FastMix or the DyNAmo Flash SYBR Green PCR Kit (Finnzymes) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Fusion of DNA-binding peptide to Tbr DNA pol results in lower specificity of the DyNAmo kit which is evident in false positive results for no template control (NTC) reactions. Chemically modified polymerase produces delayed Cts and lower signal strength compared to AccuStart™ Taq. Cycling conditions: Finnzymes: 95°C, 7 min followed by 40 cycles of 95°C, 10s; 60°C, 20s PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

HOME
ABOUT US
BRANDS
TESTIMONIALS
CONTACT US