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Molecular Biology

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0.2mL 96-Well PCR Plate, Half Skirt, Clear
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0.2mL 96-Well PCR Plate, No Skirt, Flat Top, Clear
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0.2mL 96-Well PCR Plate, Skirt, Clear
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PCR 8 Strip Tubes Caps
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PCR Tube Strips with Caps Strips
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X-Series Filter Tips
- Cat # PTF-10ul
- Cat # PTF-20ul
- Cat # PTF-100ul
- Cat # PTF-200ul
- Cat # PTF-1000ul
- Sold In: Case (4800 Tips/Case)
- Pack Size: 960 Tips/Pack
- Multi-channel pipette compatible: Yes
- Universal Fit for research-grade pipettors: Yes
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PCR Tubes (10,000 Tubes)
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PCR 8 Strip Tubes
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Automation Pipette Tips – Tecan
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HighPrep™ RNA Elite Clean-up System
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HighPrep™ Viral DNA/RNA Extraction Kit
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HighPrep™ PCR Clean-up System
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1.5 ml Micro-centrifuge Tubes
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KingFisher Flex Consumables
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10ml Serological Pipettes
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25ml Serological Pipettes
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Comparison to SYBR GreenER, ADAR

PerfeCTa SYBR Green FastMix comparison to SYBR GreenER qPCR SuperMix

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 1 pg) using PerfeCTa™ SYBR Green FastMix or the SYBR GreenER qPCR SuperMix (Invitrogen) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Replicate CT values are shown on the standard curve (Panel A, inset). Cycling conditions: Invitrogenn: 95°C, 10 min followed by 40 cycles of 95°C, 10s; 60°C, 60s; PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s. PerfeCTa SYBR Green FastMix amplified the ADAR gene with higher efficiency and greater sensitivity. All replicate reactions for SYBR GreenER qPCR SuperMix failed to amplify ADAR from 1 pg of cDNA.

Comparison to Takara, ADAR

PerfeCTa SYBR Green FastMix Comparison to SYBR PreMix Ex Taq

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa™ SYBR Green FastMix or SYBR PreMix Ex Taq™ (Takara) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. PerfeCTa™ SYBR Green FastMix produces higher fluorescent signal and detection of equal target amounts at earlier Cts. Cycling conditions for both kits: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

Comparison to Finnzymes, ADAR

PerfeCTa SYBR Green FastMix Comparison to DyNAmo Flash SYBR Green PCR Kit

RNA-specific adenosine deaminase (ADAR) was amplified from log-fold dilutions of total HeLa cell cDNA (100 ng to 10 pg) using PerfeCTa SYBR Green FastMix or the DyNAmo Flash SYBR Green PCR Kit (Finnzymes) according to each manufacturers protocol. Averaged plots for quadruplicate reactions for each input quantity are shown. Fusion of DNA-binding peptide to Tbr DNA pol results in lower specificity of the DyNAmo kit which is evident in false positive results for no template control (NTC) reactions. Chemically modified polymerase produces delayed Cts and lower signal strength compared to AccuStart™ Taq. Cycling conditions: Finnzymes: 95°C, 7 min followed by 40 cycles of 95°C, 10s; 60°C, 20s PerfeCTa™ SYBR Green FastMix: 95°C, 20s followed by 40 cycles of 95°C, 1s; 60°C, 20s

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