Quick-RNA Plant Kit

Quick-RNA Plant Kit

Cat # Name Size
R2024 Quick-RNA Plant Miniprep Kit 50 preps

Documents

Protocol

Data Sheet

SDS (MSDS)

Categories: , ,

Description

R2024

Highlights

High Quality: Extract intact high quality total RNA (including small/micro RNAs) from a wide variety of plant samples.

Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.

Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.

Description

The Quick-RNA Plant Miniprep Kit provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the Quick-RNA Plant Miniprep features a specially formulated RNA Lysis Buffer. The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin III-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.

Equipment

Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)

Purity

RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.

Sample Source

Leaves, stems, buds, flowers, fruit, seeds, etc.

Size Range

Total RNA ≥ 17 nt

Yield

50 µg RNA (binding capacity), ≥25 µl (elution volume)

Kt Components

Cat # Name Size
W1001-4 DNase/RNase-Free Water 4 ml
R1003-3-12 RNA Wash Buffer 12 ml
R1060-1-50 RNA Lysis Buffer 50 ml
R1060-2-25 RNA Prep Buffer 25 ml
C1001-50 Collection Tubes 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
C1058-50 Zymo-Spin III-HRC Filters 50 Pack
S6003-50 ZR BashingBead Lysis Tubes (2 mm) 50 Tubes
C1078-50 Zymo-Spin IICR Columns 50 Pack