5-Fluoroorotic Acid (5-FOA)

5-Fluoroorotic Acid (5-FOA)

Cat#NameSize
F9001-15-Fluoroorotic Acid (powder)1 g
F9001-55-Fluoroorotic Acid (powder)5 g
F9003100X 5-Fluoroorotic Acid (Liquid)10 ml

Documents

Protocol:

SDS (MSDS): F9001

SDS (MSDS): F9003

Description

Highlights

  •  Yeast Genetic Counter-Selection Agent: Commonly used for curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens.
  •  Convenient: Available as a pure powder or ready-to-use solution in DMSO
  •  Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison.

Description

Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection. The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and synthetic 5-FOA monohydrate powder. Both have been tested and validated on the basis of counter selection activity.

Format

Synthetic 5-FOA monohydrate powder or 100X liquid (100 mg/ml in DMSO)

Molecular Weight

174 g/mol

Product Storage

Store at -20°C

Purity

≥ 98% (estimated) by TLC, melting point, and lot comparison

Solubility

50 mg in 1 ml (1:1 1:1 NH4 OH:H2O) with gentle heating; >100 mg/ml in DMSO

Structure

C5H3FN2O4• H2O

  • Catalog#: F9001-1 /F9001-5 /F9003
  • Package Length (in Inches): 1
  • Package Width (in Inches): 1
  • Package Height (in Inches): 2.4
  • Package Weight (in Pounds): 0.1 /0.2 /0.1
  • Size: 1 g /5 g /10 ml
  • Unit Standard: Metric
  • Volume Units: Milliliters