5-Methyl dCTP

5-Methyl dCTP

Cat#NameSize
D1035D103510 mM

Documents

Protocol:

SDS (MSDS):

Description

Highlights

  •  Ready-to-use, ultra pure 5-Methylcytosine dCTP; >99% trisphosphate as determined by HPLC analysis.
  •  Readily incorporates into PCR amplicons with ZymoTaq or other DNA polymerases.
  •  Free of endo/exo-deoxyribonucleases, ribonucleases, and phosphatases.

Description

5-Methyl dCTP can be used in nucleotide mixtures to generate PCR amplicons using ZymoTaq, QuestTaq, or other DNA polymerases.

Applicable For

CPCR amplicons containing 5-methylcytosine are useful in a variety of applications such as studying 5-methylcytosine binding/interacting proteins or defining methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine

Concentration

5-Methylcytosine dNTP Mix: 1 µmol/100 µl as a [10 mM] solution.

Sample Storage

5-Methylcytosine dNTP Mix is guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.

  • Catalog#: D1035
  • Package Length (in Inches): 1.8
  • Package Width (in Inches): 0.4
  • Package Height (in Inches): 0.4
  • Package Weight (in Pounds): 0.1
  • Size: 10mM
  • Unit Standard: Metric
  • Volume Units: Milliliters