Documents
Description
Highlights
- Effectively preserves DNA and RNA in urine at ambient temperatures
- Facilitates pelleting of urine nucleic acids from large volume urine samples for nucleic acids purification
- Microbial inactivation
Description
DNA/RNA Shield Lysis and Collection tubes are microbial lysis tubes and bead beating tubes designed for the streamlined collection, transport, and processing of biological specimen. The microbial lysis tubes and bead beating tubes are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization. Bead sizes include 0.5mm & 0.1mm (R1103 and R1104) and 2.0mm (R1105).
Applicable For
Next-Generation Sequencing, qPCR, microarray
Device Specs
2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 – 0.5 mm & 0.1 mm beads, R1105 – 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint.
Device Storage
Ambient temperature => 2 years
Sample Collection
10% (v/v or w/v)
Sample Source
Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
Sample Stability
RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q3: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q4: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
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99 / 100 Bioz Stars |
Presence of the point mutations Val1016Gly in the voltage-gated sodium c…
Parasit Vectors – Published 28 Jan 2019
The paddles with eggs were immersed in trays of water and were reared to adult stage.. Subsequently, the mosquitoes were placed in 1.5 ml conical tubes with 500 μl of DNA/RNA Shield™ (Zymo Research, Irvine, CA, USA) and stored at -80 °C.. The genomic DNA of the mosquitoes was isolated with a ZR Viral DNA/RNA Kit™ (Zymo Research) according to the manufacturer’s instructions.
A quasi-integral controller for adaptation of genetic modules to variabl…
Nat Commun – Published 21 Dec 2018
least 120,000 singlet events were collected for data analysis with the FlowJo v10 (FlowJo, LLC) and the CFlowPlus v1.0.264.14 (Becton Dickinson) softwares. . At the end of the last batch of an experiment (OD600nm ~0.05), 200 μL E. coli cells were lysed with 1150 μL DNA/RNA Shield 2× concentrate solution (Zymo Research R1200-125).. Total RNA was extracted with Quick-RNA Microprep Kit (Zymo Research, R1051) and quantified with the nanophotometer (Implen, P330).’
Phylogenetic analysis of West Nile Virus in Maricopa County, Arizona: Ev…
PLoS One – Published 26 Nov 2018
(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Zika virus sequencing. . Prior to transporting the samples, an equal part of DNA/RNA ShieldTM 2X Concentrate (Zymo Research) was added to each mosquito pool to stabilize the RNA and inactivate WNV.. The pools consisted of mosquitos in approximately 1.3 ml of TE buffer (Invitrogen, AM9858).
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
– Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
– Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
– Maha AE. (University of Florida)
“We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.”
– Mariska B. (University of Amsterdam)
- Catalog#: R1100-8-S/ R1103S / R1102 / R1103 / R1104 /R1105
- Package Length (in Inches): 0 /5
- Package Width (in Inches): 0 /7
- Package Height (in Inches): 0 /1
- Package Weight (in Pounds): 0.6 /0.9
- Size: Sample /50 Pack
- Unit Standard: Metric
- Volume Units: Milliliters
Highlights
- Effectively preserves DNA and RNA in urine at ambient temperatures
- Facilitates pelleting of urine nucleic acids from large volume urine samples for nucleic acids purification
- Microbial inactivation
Description
DNA/RNA Shield Lysis and Collection tubes are microbial lysis tubes and bead beating tubes designed for the streamlined collection, transport, and processing of biological specimen. The microbial lysis tubes and bead beating tubes are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization. Bead sizes include 0.5mm & 0.1mm (R1103 and R1104) and 2.0mm (R1105).
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
– Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
– Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
– Maha AE. (University of Florida)
“We extracted RNA from cells suspended in DNA/RNA Shield with the Direct-zol miniprep set from Zymo (using Trizol). It worked perfect for us.”
– Mariska B. (University of Amsterdam)
Applicable For
Next-Generation Sequencing, qPCR, microarray
Device Specs
2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 – 0.5 mm & 0.1 mm beads, R1105 – 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint.
Device Storage
Ambient temperature => 2 years
Sample Collection
10% (v/v or w/v)
Sample Source
Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
Sample Stability
RNA: Ambient temperature (4°C-25°C) > 1 month
DNA: Ambient temperature (4°C-25°C) > 2 years
DNA & RNA: Frozen (< -20°C): Indefinitely
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q3: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q4: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
|
99 / 100 Bioz Stars |
Presence of the point mutations Val1016Gly in the voltage-gated sodium c…
Parasit Vectors – Published 28 Jan 2019
The paddles with eggs were immersed in trays of water and were reared to adult stage.. Subsequently, the mosquitoes were placed in 1.5 ml conical tubes with 500 μl of DNA/RNA Shield™ (Zymo Research, Irvine, CA, USA) and stored at -80 °C.. The genomic DNA of the mosquitoes was isolated with a ZR Viral DNA/RNA Kit™ (Zymo Research) according to the manufacturer’s instructions.
A quasi-integral controller for adaptation of genetic modules to variabl…
Nat Commun – Published 21 Dec 2018
least 120,000 singlet events were collected for data analysis with the FlowJo v10 (FlowJo, LLC) and the CFlowPlus v1.0.264.14 (Becton Dickinson) softwares. . At the end of the last batch of an experiment (OD600nm ~0.05), 200 μL E. coli cells were lysed with 1150 μL DNA/RNA Shield 2× concentrate solution (Zymo Research R1200-125).. Total RNA was extracted with Quick-RNA Microprep Kit (Zymo Research, R1051) and quantified with the nanophotometer (Implen, P330).’
Phylogenetic analysis of West Nile Virus in Maricopa County, Arizona: Ev…
PLoS One – Published 26 Nov 2018
(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Zika virus sequencing. . Prior to transporting the samples, an equal part of DNA/RNA ShieldTM 2X Concentrate (Zymo Research) was added to each mosquito pool to stabilize the RNA and inactivate WNV.. The pools consisted of mosquitos in approximately 1.3 ml of TE buffer (Invitrogen, AM9858).
- Catalog#: R1100-8-S/ R1103S / R1102 / R1103 / R1104 /R1105
- Package Length (in Inches): 0 /5
- Package Width (in Inches): 0 /7
- Package Height (in Inches): 0 /1
- Package Weight (in Pounds): 0.6 /0.9
- Size: Sample /50 Pack
- Unit Standard: Metric
- Volume Units: Milliliters
