Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21)
| Cat# | Name | Size |
|---|---|---|
| A3002-15 | Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21) | 15 µl |
| A3002-200 | Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21) | 200 µl |
| A3002-30 | Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21) | 30 µl |
| A3002-50 | Anti-5-Methylcytosine Monoclonal Antibody (Clone 7D21) | 50 µl |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- Specifically binds to 5-methylcytosine in ssDNA context.
- No detectable cross-reactivity with non-methylated cytosine.
Description
The mouse Anti-5-Methylcytosine Monoclonal Antibody specifically recognizes methylated cytosines. The antibody binds to 5-mC in single-stranded DNA, with no detectable cross-reactivity to non-methylated or hydoxymethylated cytosines. This product is ideal for immuno-based assays such as methylated DNA Immunoprecipitation (MeDIP), ELISA and dot blot..
Buffer Solution
Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative.
Clone
7D21
Isotype
IgG1 (κ light chain)
Protein Concentration
0.5 µg/µl
Source
Mouse
Specificity
5-Methylcytosine in single-stranded DNA from any source including human, rat, mouse, plants, etc.
Storage
Store at -20°C with prolonged storage at -80°C. Avoid repeated freeze/thaws.
 |
95 / 100 Bioz Stars |
Integrative analysis of DNA methylation, mRNAs, and small RNAs during ma…
BMC Plant Biol – Published 15 Jun 2017
Double-stranded DNA was denatured and immunoprecipitated using an anti-5-methycytosine monoclonal antibody (Zymo Research, Orange, CA, USA), and 3’dA-tailing using the Paired-end DNA Sample Prep Kit (Illumina, San Diego, CA, USA), the DNA samples were ligated to Illumina sequencing primer adaptors. . Double-stranded DNA was denatured and immunoprecipitated using an anti-5-methycytosine monoclonal antibody (Zymo Research, Orange, CA, USA).. 220 bp to 320 bp bands were excised and purified from the immunoprecipitation gel and quantified using an Agilent 2100 Analyzer (Agilent Technologies
Identification of MicroRNA-124-3p as a Putative Epigenetic Signature of …
Neuropsychopharmacology – Published 05 Oct 2016
Two micrograms of total gDNA isolated from rat PFC was treated with RNaseA and sheared to generate a random fragment length of ~400 bp.. Fragmented gDNA was first denatured and then was conjugated using Protein G magnetic beads (EMD Millipore) and 5 μg monoclonal 5-methyl cytosine antibody (Zymo Research, Irvine, CA).. A portion of (10%) untreated sonicated denatured gDNA was kept separately as input control.’
- Catalog#: A3002-15 /A3002-200 /A3002-30 A3002-50
- Package Length (in Inches): 1.8
- Package Width (in Inches): 0.4
- Package Height (in Inches): 0.4
- Package Weight (in Pounds): 0.1
- Size: 15 µl /200 µl /30 µl /50 µl
- Unit Standard: Metric
- Volume Units: Milliliters
