BlasTaq™ HotStart DNA Polymerase

BlasTaq™ HotStart DNA Polymerase

Cat# Unit
G595400 rxn

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Description

Description

Eliminate non-specific amplification and primer-dimer formation!


BlasTaq™
 HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

 

Product Features:

  • No non-specific amplification or primer-dimers
  • Improves yield of desired product
  • Simplifies reaction setup
  • Earn 2X the rewards points

 

PRODUCT COMPONENT QUANTITY
BlasTaq™ HotStart DNA Polymerase 400 rxn (200 µl)
5X BlasTaq™ Buffer 1 2 x 1.0 ml

 

SKU G595
Storage Condition

Store at -20°C.

Unit quantity 400 rxn
  • Chung, G et al. “CHL-1 provides an essential function affecting cell proliferation and chromosome stability in Caenorhabditis elegans” DNA Repair (Amst.) 10 (11):1174-1182 (2011). DOI: 10.1016/j.dnarep.2011.09.011. PubMed: 21968058. Application: PCR.