DNA Clean & Concentrator-5
| Cat# | Name | Size |
|---|---|---|
| D4004 | DNA Clean & Concentrator-5 (Uncapped) | 200 Preps |
| D4003 | DNA Clean & Concentrator-5 (Uncapped) | 50 Preps |
| D4014 | DNA Clean & Concentrator-5 (Capped) | 200 Preps |
| D4013 | DNA Clean & Concentrator-5 (Capped) | 50 Preps |
| D4003T | DNA Clean & Concentrator-5 (Uncapped) | 10 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
- Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Detergent Tolerance
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
Elution Volume
≥ 6 µl
Equipment
Microcentrifuge
Purity
A260/A280 > 1.8
Sample Source
DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.
Size Range
50 bp to 23 kb
Yield
≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
Q1: What is the difference between capped and uncapped?
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Q2: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Q3: How many times can columns be reloaded?
Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How can I process naked DNA stored in DNA/RNA Shield?
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
Q6: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
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99 / 100 Bioz Stars |
SMARCA4 loss is synthetic lethal with CDK4/6 inhibition in non-small cel…
Nat Commun – Published 4 Feb 2019
After cleaning with Zymo DNA Clean and Concentrator-5 Kit (Zymo Research cat. no. D4014), eluted DNA was pre-amplified for 5 cycles using NEBNext 2x MasterMix (NEB cat. no. M0541).. Additional amplification cycles were added based on qPCR amplification.
SLFN11 blocks stressed replication forks independently of ATR
Mol Cell – Published 01 Feb 2019
irectly following transposition, the sample was purified using a DNA Clean and Concentrator-5 (ZYMO RESEARCH) and eluted with 25 μl of DNA elution buffer.
TaME-seq: An efficient sequencing approach for characterisation of HPV g…
Sci Rep – Published 24 Jan 2019
agmented DNA was purified using DNA Clean & Concentrator™-5 columns (Zymo Research, Irvine, CA) according the manufacturer’s instructions or ZR-96 DNA Clean & Concentrator™-5 plates (Zymo Research, Irvine, CA) according to the Nextera® DNA Library Prep Reference Guide (15027987 v01) before PCR amplification for target enrichment.. Amplification was performed using Qiagen Multiplex PCR Master mix (Qiagen, Hilden, Germany) according to the manufacturer’s instructions.
| Cat # | Name | Size |
|---|---|---|
| D3004-4-1 | DNA Elution Buffer | 1 ml |
| D3004-4-4 | DNA Elution Buffer | 4 ml |
| D4003-1-25 | DNA Binding Buffer | 25 ml |
| D4003-1-L | DNA Binding Buffer | 50 ml |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1001-500 | Collection Tubes | 500 Pack |
| C1001-1000 | Collection Tubes | 1000 Pack |
| C1003-50 | Zymo-Spin I Columns | 50 Pack |
| C1003-250 | Zymo-Spin I Columns | 250 Pack |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
| C1004-250 | Zymo-Spin IC Columns | 250 Pack |
| D4004-1-L | DNA Binding Buffer | 100 ml |
- Catalog#: D4004 /D4003 /D4014 /D4013 /D4003T
- Package Length (in Inches): 9 /7 /9 /7 /2.5
- Package Width (in Inches): 6.5 /5 /6.5 /5 /2.2
- Package Height (in Inches): 6 /4.5 /6 /4.5 /1
- Package Weight (in Pounds): 2 /0.7 /2.1 /0.8
- Size: 200 Preps /50 Preps /200 Preps /50 Preps /10 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
