DNA Clean & Concentrator-5

DNA Clean & Concentrator-5

Cat#NameSize
D4013DNA Clean & Concentrator-5 (Capped)50 Preps

Documents

Protocol:

Datasheet:

SDS (MSDS)

Categories: , ,

Description

Highlights

  • Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
  • Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
  • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Description

The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.

Detergent Tolerance

≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS

Elution Volume

≥ 6 µl

Equipment

Microcentrifuge

Purity

A260/A280 > 1.8

Sample Source

DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.

Size Range

50 bp to 23 kb

Yield

≤ 5 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.

Q1: What is the difference between capped and uncapped?

The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.

Q2: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.

Q3: How many times can columns be reloaded?

Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How can I process naked DNA stored in DNA/RNA Shield?

Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.

Q6: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q8: Can I clean up DNA from agarose gel?

We have a Zymoclean Gel DNA Recovery Kit that has chemistry compatible with agarose gel. However, if you have the DNA Clean & Concentrator kits on hand, you can clean up DNA from gel.

Cat#NameSize
D3004-4-1DNA Elution Buffer1 ml
D3004-4-4DNA Elution Buffer4 ml
D4003-1-25DNA Binding Buffer25 ml
D4003-1-LDNA Binding Buffer50 ml
D4003-2-24DNA Wash Buffer (Concentrate)24 ml
D4003-2-6DNA Wash Buffer (Concentrate)6 ml
C1001-50Collection Tubes50 Pack
C1001-500Collection Tubes500 Pack
C1001-1000Collection Tubes1000 Pack
C1003-50Zymo-Spin I Columns50 Pack
C1003-250Zymo-Spin I Columns250 Pack
C1004-50Zymo-Spin IC Columns50 Pack
C1004-250Zymo-Spin IC Columns250 Pack
D4004-1-LDNA Binding Buffer100 ml