DNase I Set
| Cat # | Name | Size |
|---|---|---|
| E1010 | DNase I Set | 250 U |
Documents
SDS (MSDS):
Datasheet:
Description
Highlights
- Lyophilized enzyme provided with DNA Digestion Buffer
Description
DNase I (RNase-free) cuts both double-stranded and single-stranded DNA, producing 3′-OH oligonucleotides. It is typically used for selectively degrading DNA in the presence of RNA. This DNase is suited for applications such as nick translation, production of random fragments, cleavage of genomic DNA for footprinting, removal of DNA template after in vitro transcription, and removal of DNA from RNA samples prior to applications such as RT-PCR. It is compatible with all of our RNA kits featuring in-column DNase digestion. The DNase I Set features lyophilized enzyme and DNA Digestion Buffer.
Concentration
1 U/µl
Heat inactivation
Add a final concentration of 5 mM EDTA, then incubate at 65°C for 10 min..
Included
One unit (U) is defined as the amount of enzyme required to degrade 1 µg λ DNA completely in 10 minutes at 37°C in a 50 µl reaction volume (40 mM Tris-HCl, pH 8.0, 10 mM NaCl, 6 mM MgCl2, and 10 mM CaCl2). One unit of enzyme is equivalent to one Kunitz unit.
Resuspension Volume
275 µl
Sample Source
Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
Size
250 U
| Cat # | Name | Size |
|---|---|---|
| E1010-1-4 | DNA Digestion Buffer | 4 mL |
