EZ RNA Methylation Kit
| Cat# | Name | Size |
|---|---|---|
| R5001 | EZ RNA Methylation Kit | 50 Preps |
| R5002 | EZ RNA Methylation Kit | 200 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Hightlights
- Specifically optimized for complete conversion of non-methylated cytosines in RNA.
- Ideal for all RNA inputs.
- Complete conversion of RNA in as little as 1 hour.
Description
The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated. The innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.
Conversion
> 99%
Elution Volume
≥ 10 µl
Equipment
Thermocycler with heated lid and microcentrifuge.
Input
0.5 – 1 µg of RNA.
Processing Time
50 Minutes
Purity
Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Recovery
> 80%
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88 / 100 Bioz Stars |
Dnmt2 mediates intergenerational transmission of paternally acquired met…
Nat Cell Biol – Published 25 Oct 2018
Sperm RNAs were prepared as previously mentioned.. Bisulfite conversion was performed using the EZ RNA MethylationTM Kit (Zymo Research, catalog number: R5001) according to manufacturer’s protocol.. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously
Statistically robust methylation calling for whole-transcriptome bisulfi…
Genome Res – Published 1 Sep 2017
454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research).
Statistically robust methylation calling for whole-transcriptome bisulfi…
Genome Res – Published 1 Sep 2017
(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Thirty micrograms of total RNA was fractionated into a long ( > 200 nt) and small ( < 200 nt) RNA fraction and depleted for rRNA as indicated.. Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research).. After stepwise RNA end repair and further purification, cDNA synthesis and library preparation was carried out using the NEBNext Small RNA Library
| Cat # | Name | Size |
|---|---|---|
| W1001-1 | DNase/RNase-Free Water | 1 ml |
| W1001-4 | DNase/RNase-Free Water | 4 ml |
| R1003-3-12 | RNA Wash Buffer | 12 ml |
| R1003-3-24 | RNA Wash Buffer | 24 ml |
| R1013-2-100 | RNA Binding Buffer | 100 ml |
| R1013-2-25 | RNA Binding Buffer | 25 ml |
| R5001-1-1 | RNA Conversion Reagent | 1.5 ml |
| R5001-3-10 | RNA Desulphonation Buffer | 10 ml |
| R5001-3-40 | RNA Desulphonation Buffer | 40 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
- Catalog#: R5001 /R5002
- Package Length (in Inches): 7 /9
- Package Width (in Inches): 5 /6.5
- Package Height (in Inches): 4.5 /6
- Package Weight (in Pounds): 0.8 /2.1
- Size: 50 Prep /200 Prep
- Unit Standard: Metric
- Volume Units: Milliliters
