Oligo Clean & Concentrator

Oligo Clean & Concentrator

Cat#NameSize
D4060Oligo Clean & Concentrator50 Preps
D4061Oligo Clean & Concentrator200 Preps

Documents

Protocol:

Datasheet:

SDS (MSDS): D4060

SDS (MSDS): D4061

Categories: , ,

Description

Highlights

  • Fast & Simple: Clean and concentrate DNA and RNA oligonucleotides in 2 minutes.
  • Ultra-Pure: Ready for hybridization, Next-Gen Sequencing, PCR, ligations, etc.
  • Complete removal: Eliminate dyes, salts, enzymes, nucleotides, and short oligos.

Description

The Oligo Clean & Concentrator provides a streamlined method for efficient recovery and clean-up of DNA/RNA fragments and oligonucletides ≥ 16 nt from labeling (radioactive, biotin, DIG, etc.) and other enzymatic reactions. Unincorporated nucleotides, short oligos, dyes, enzymes, and salts are effectively removed by the clean-up procedure. DNA/RNA is washed and concentrated into a small volume of water. Purified DNA/RNA, available in just 2 minutes, is suitable for hybridization, gel shift assays, enzymatic reactions, ligation, sequencing, microarray analysis, etc.

Applicable For

Single-stranded and double-stranded DNA and RNA

Elution Volume

≥ 6 µl

Equipment

Microcentrifuge

Purity

A260/A280 > 1.8, A260/A230 > 1.8

Sample Source

Enzymatic reaction mixtures containing oligonucleotides ≥ 16 nt (radioactive-, biotin-, DIG-labeled, etc.)

Size Range

For oligonucleotides ≥ 16 nt, up to 23 kb

Yield

10 µg of ssDNA/RNA or 5 µg of dsDNA with a typical recovery > 90%

99 / 100
Bioz Stars

Repurposing CRISPR-Cas12b for mammalian genome engineering

Cell Discov – Published 27 Nov 2018

Transcribed RNAs were purified using Oligo Clean & ConcentratorTM (ZYMO Research) and quantitated on NanoDropTM 2000 (Thermo Fisher) were in vitro-transcribed using HiSribeTM T7 Quick High Yield RNA Synthesis Kit (NEB) and PCR-amplified DNA templates carrying a T7 promoter sequence. . Transcribed RNAs were purified using Oligo Clean & ConcentratorTM (ZYMO Research) and quantitated on NanoDropTM 2000 (Thermo Fisher).. Primers used for template preparation are listed in Supplementary Table .

Mettl3-mediated m6A RNA methylation regulates the fate of bone marrow me…

Nat Commun -Published 14 Nov 2018

he mRNA fragments were purified with Oligo Clean & ConcentratorTM (Zymoresearch D4061) and then subjected to immunoprecipitation with α-m6 A antibody (1:100, Synaptic Systems, Cat No: 202003).

Regulatory Targets of the Response Regulator RR_1586 from Clostridioide…

J Bacteriol – Published 6 Nov 2018

EmeraldAmp GT master mix (Clontech) was used for all PCRs unless explicitly stated.. ZymoPURE midiprep, DNA Clean & Concentrator 5 (DNA C & C), and Oligo Clean & Concentrator (Oligo C & C) kits were purchased from Zymo Research.. Restriction enzymes, ligases, and RecA were purchased from NEB.

Cat # Name Size
D4060-1-10 Oligo Binding Buffer 10 ml
D4060-1-40 Oligo Binding Buffer 40 ml
D4003-2-24 DNA Wash Buffer (Concentrate) 24 ml
D4003-2-48 DNA Wash Buffer (Concentrate) 48 ml
C1001-50 Collection Tubes 50 Pack
C1001-500 Collection Tubes 500 Pack
C1001-1000 Collection Tubes 1000 Pack
C1004-50 Zymo-Spin IC Columns 50 Pack
C1004-250 Zymo-Spin IC Columns 250 Pack
  • Catalog#: D4060 / D4061
  • Package Length (in Inches): 7 / 13
  • Package Width (in Inches): 5 /8
  • Package Height (in Inches): 4.5 / 6.5
  • Package Weight (in Pounds): 0.5 / 2.6
  • Size: 50 Preps / 200 Preps
  • Unit Standard: Metric
  • Volume Units: Milliliters