Pinpoint Slide RNA Isolation System
| Cat # | Name | Size |
| R1003 | Pinpoint Slide RNA Isolation System I | 50 preps |
| R1007 | Pinpoint Slide RNA Isolation System II | 50 preps |
Documents
Protcol: R1003
Protcol: R1007
SDS (MSDS): R1003
SDS (MSDS): R1007
Description
R1003 / R1007
Highlights
- High Quality: Allows for the isolation of total RNA from fresh tissue sections (System I) or FFPE tissues (System II) on glass slides.
- Simple: Pinpoint tissue sampling technology with a one-step RNA extraction/purification method.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
Description
The Pinpoint Slide RNA Isolation Systems I and II are innovative products for the isolation of RNA from any targeted area of fresh, paraffin-fixed, or paraffin-embedded tissues sectioned onto a glass slide. The system combines powerful Pinpoint tissue sampling methodology, a unique single-step RNA extraction/binding buffer, and Zymo-Spin column purification technology to yield high quality RNA. Unlike current UV-based methods, this product makes isolation of tissue RNA simple and quick. No expensive specialized equipment is needed. Eluted RNA is well suited for subsequent RNA analyses including RT-PCR.
Equipment
Microcentrifuge
Purity
RNA is ready for RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Cells and tissue sections from fresh, paraffin-fixed, or paraffin-embedded tissue sections on glass slides
Size Range
Total RNA ≥ 17 nt
Yield
10 µg RNA (binding capacity), ≥6 µl (elution volume)
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93 / 100 Bioz Stars |
Chromatin accessibility programs CD8 T cell memory
J Immunol Published 15 Mar 2018
‘Cells were fixed in 1% paraformaldehyde and virus-specific P14 cells were sorted by FACS using markers described above.. RNA was prepared using the Pinpoint Slide RNA Isolation System (Zymo Research).. ATAC-seq libraries were generated from 104 cells for each sample, sequenced, and data processed as detailed previously ( ).’
KIF7 attenuates prostate tumor growth through LKB1-mediated AKT inhibiti…
Oncotarget Published 26 Apr 2017
‘Cells were treated with paclitaxel from 0 to 30 nM for 3 days.. FFPE PCa and normal tissue blocks were microdissected for RNA extraction using a Pinpoint™ Slide RNA Isolation System II kit (Zymo Research, Orange, CA).. RNA isolation from cells, cDNA synthesis and quantitative real-time polymerase chain reaction (qPCR) were performed as described [ ].’
A PGC1α-mediated transcriptional axis suppresses melanoma metastasis
’18S rRNA was used as internal control.. For cells from paraffin-embedded tissue sections, Pinpoint™ Slide RNA Isolation System II (Zymo Research) was used to extract RNAs.. Cells with different mitochondrial contents were sorted based on the labeling of MitoTracker Green (Invitrogen).’
