Proteinase K w/ Storage Buffer Set
| Cat # | Name | Size |
|---|---|---|
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg |
| D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg |
Documents
SDS (MSDS): Storage Buffer
SDS (MSDS): Proteinase K
Description
Highlights
- Lyophilized enzyme provided with Proteinase K Storage Buffer.
Description
Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.
Enzyme Commission Number
(EC 3.4.21.64)
pH and Temperature Range
2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 – 0.5 mm & 0.1 mm beads, R1105 – 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint.
Source
Engyodontium album
Specific Activity
10% (v/v or w/v)
Sample Source
> 30 units/mg protein
Unit Definition
One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.
