qScript XLT 1-Step RT-qPCR ToughMix

qScript XLT 1-Step RT-qPCR ToughMix

Products Kit Size Order Info
qScript XLT 1-Step RT-qPCR ToughMix100 x 20 μL rxns (1 x 1 mL)Order (95132-100)
500 x 20 μL rxns (5 x 1 mL)Order (95132-500)
2000 x 20 μL rxns (1 x 20 mL)Order (95132-02K)

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Description

Tough-tested 1-step real time PCR

Features & Benefits

  • Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
  • Flexible – Use fast or standard qPCR cycling conditions
  • Broad dynamic range- Reliable data from your precious samples every time
  • Multiplexing enabled – Supports highly sensitive detection for up to four targets

qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.

This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.

Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

  • 2X concentrated One-Step Master Mix containing:
  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • qScript XLT reverse transcriptase
  • RNase inhibitor protein
  • AccuStart II Taq DNA Polymerase
  • Inert AccuVue™ plate loading dye
  • Proprietary enzyme stabilizers and performance-enhancing additives
    • Titrated reference dye (if applicable)

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5