Circulating tumor DNA as a liquid biopsy target for detection of pancrea…

World J Gastroenterol Published 14 Oct 2016

‘standardize preanalytical processes for cfDNA analysis, such as blood sample acquisition, plasma separation, sample storage, cfDNA extraction and quantification. . Recently, there are an increasing number of new products for cfDNA processing including blood collection tubes [e.g ., Cell-Free DNA BCT® (Streck) and Cell-Free DNA Collection Tube (Roche)] and cfDNA extraction kits [e.g ., Quick-cfDNA™ Serum & Plasma Kit (Zymo Research), Maxwell® RSC ccfDNA Plasma Kit (Promega), and MagMAX™ Cell-Free DNA Isolation Kit (Thermo Fisher Scientific)].. For sequencing of cfDNA, new library preparation kits optimized for small amounts of fragmented DNA, such as Accel-NGS® DNA Library Kits (Swift Biosciences) ‘