Quick-cfRNA Serum & Plasma Kit
| Cat # | Name | Size |
|---|---|---|
| R1059 | Quick-cfRNA Serum & Plasma Kit | 50 preps |
Documents
Protocol:
SDS (MSDS):
White paper:
Description
R1059
Highlights
Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
Description
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
Elution Volume
cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time
Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity
High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment
Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Type
Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size
RNA ≥ 17 nt.
Yield
Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.
Q1: Can saliva be used as sample input?
Saliva must be cleared to remove debris/mucus (12,000xg for 15 min at room temperature). Up to 1 ml cleared salvia can be used per extraction.
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86/ 100 Bioz Stars |
Circulating tumor DNA as a liquid biopsy target for detection of pancrea…
World J Gastroenterol Published 14 Oct 2016
‘standardize preanalytical processes for cfDNA analysis, such as blood sample acquisition, plasma separation, sample storage, cfDNA extraction and quantification. . Recently, there are an increasing number of new products for cfDNA processing including blood collection tubes [e.g ., Cell-Free DNA BCT® (Streck) and Cell-Free DNA Collection Tube (Roche)] and cfDNA extraction kits [e.g ., Quick-cfDNA™ Serum & Plasma Kit (Zymo Research), Maxwell® RSC ccfDNA Plasma Kit (Promega), and MagMAX™ Cell-Free DNA Isolation Kit (Thermo Fisher Scientific)].. For sequencing of cfDNA, new library preparation kits optimized for small amounts of fragmented DNA, such as Accel-NGS® DNA Library Kits (Swift Biosciences) ‘
