Quick-DNA FFPE Miniprep
| Cat# | Name | Size |
|---|---|---|
| D3067 | Quick-DNA FFPE Miniprep | 50 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- Streamlined purification of high-quality FFPE tissue DNA that is ideal for PCR, Next-Gen library prep, enzymatic manipulations, etc.
- Size selection technology; recover total DNA >50 bp or >500 bp.
Description
The Quick-DNA FFPE Kit provides a simple and reliable method for high yield/quality DNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples and sections. The unique chemistries of the product have been optimized for maximum recovery of non-crosslinked, ultra-pure DNA without RNA contamination. Simply digest deparaffinized tissues using the provided Proteinase K, heat, and then purify the DNA with the Zymo-Spin columns in the kit. DNA >50 bp or >500 bp can be selectively isolated by altering the lysis buffer conditions as described in the protocol. PCR inhibitors are effectively removed during the isolation procedure and eluted DNA is ideal for PCR, Next-Gen library prep, enzymatic manipulation, etc.
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment
Microcentrifuge, heat block/bath (55°C and 90°C), isopropanol, beta-mercaptoethanol (optional).
Processing Volume
Tissue – ≤25 mg or (4) 20 µm thick with a surface area of 20 mm2
Purity
Typical A260/A280 ≥ 1.8
Sample Source
Tissue from paraffin block or tissue sections. Compatible with fresh/frozen tissue specimens
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
> 50 bp
Type
Total DNA
Yield
Up to 25 µg total DNA can be eluted into ≥ 50 µl.
| Cat # | Name | Size |
|---|---|---|
| D3050-1-5 | 2X Digestion Buffer – 5 mL | 5 mL |
| C1011-50 | Zymo-Spin IIC Columns | 50 Pack |
| C1001-50 | Collection Tubes | 50 Pack |
| D3067-1-20 | Deparaffinization Solution | 20 ml |
| D3067-2-25 | Genomic DNA Wash 1 | 25 ml |
| D3067-3-12 | Genomic DNA Wash 2 | 12 ml |
| D3004-1-50 | Genomic Lysis Buffer | 50 ml |
| D3004-4-10 | DNA Elution Buffer | 10 ml |
- Catalog#: D3067
- Package Length (in Inches): 3.5
- Package Width (in Inches): 2.6
- Package Height (in Inches): 2.4
- Package Weight (in Pounds): 1.25
- Size: 50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
