Quick-DNA Magbead Plus Kit
| Cat# | Name | Size |
|---|---|---|
| D4081 | Quick-DNA Magbead Plus Kit | 1 x 96 Preps |
| D4082 | Quick-DNA Magbead Plus Kit | 4 x 96 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- Purify high-quality DNA easily and reliably from any biological fluid, cultured/monolayer cells, or tissue sample.
- The Zymo magbead purification system ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
- The automation friendly workflow enables biological fluids, cultured/monolayer cells, or solid tissues to be processed in as little as 60 minutes for 96 preps.
Description
The Quick-DNA Magbead Plus DNA Kit is the easiest method for high throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo’s unique system allows for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultra-pure and concentrated genomic DNA (> 50 kb) in as little as 60 minutes for 96 samples. Purified DNA is ready for quantification or applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Elution Volume
≤ 100 µl
Equipment
Centrifuge fitted with a 96 well microplate carrier, heat block (55°C), 96-well magnetic rack, automated liquid handler (recommended), 2 mL 96 well plates and reagent carriers (user supplied).
Purity
A260/A280 >1.8, A260/A230 >1.8
Size Range
Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
Workflow
Utilizes a Proteinase K Digestion and magnetic binding bead technology for effective recovery of DNA from blood, cells, and tissues.
Yield
Up to 10 µg per 50 µl of MagBinding Beads used
Q1: Can I use more sample input, than what’s recommended in the protocol, by scaling up the protocol?
Custom solutions can be provided, please contact automation@zymoresearch.com.
Q2: Do you have scripts available for your automated kits and/or do you provide scripting support?
Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. The kit instruction manual includes an “automation guide” for users to script the protocol themselves and our automation support staff also provides automation troubleshooting and advice if needed. For detailed help regarding all automation questions, please contact automation@zymoresearch.com.
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99 / 100 Bioz Stars |
The design and testing of mini-barcode markers in marine lobsters
PLoS One – Published 24 Jan 2019
These samples provided 67% coverage across the different families within the lobster taxonomy.. DNA from 17 species (adults and larvae combined = 19 samples) was extracted from pereiopod tissue using the Zymo Quick-DNA Universal Kit (Zymo Research), as per the manufacturer’s protocol which was modified to include an initial incubation step at 55°C overnight.
The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline F…
Cell Rep – Published 22 Jan 2019
Distal enhancer deletion candidate lines were screened for the presence of shorter bands due to deletion.. To determine the precise mutations, genomic DNA was extracted from about 1 million cells using Quick-DNA Microprep Kit (ZYMO RESEARCH, D3021).. 1uL of the genomic DNA was used as PCR template for genotyping using Phusion High-Fidelity DNA Polymerase (NEW ENGLAND BioLabs, M0530L).
Molecular monitoring of the diversity of human pathogenic malaria specie…
Malar J – Published 15 Jan 2019
HIV-1 and HIV-2 negative blood donors were analysed for the presence of possibly undetected, low-level malaria parasites using high-sensitive qPCR assays. . DNA extraction was done manually from 180 µL whole blood using Quick-DNA Miniprep kits (Zymo Research, Irvine, USA) following manufactures’ guidelines and DNA was eluted with 50 µL Elution Buffer.. DNA samples were kept at − 20 °C prior to qPCR analysis.
| D3004-2-200 | g-DNA Wash Buffer | 200 ml |
| D3004-4-16 | DNA Elution Buffer | 16 ml |
| D3004-4-50 | DNA Elution Buffer | 50 ml |
| D3004-5-250 | DNA Pre-Wash Buffer | 250 ml |
| D3004-5-50 | DNA Pre-Wash Buffer | 50 ml |
| D4100-2-12 | MagBinding Beads | 12 ml |
| D4100-2-6 | MagBinding Beads | 6 ml |
| D4077-1-150 | Quick-DNA MagBinding Buffer | 150 ml |
| D4081-3-25 | Biofluid & Solid Tissue Buffer | 25 mL |
| D4081-3-100 | Biofluid & Solid Tissue Buffer | 100 mL |
- Catalog#: D4081 /D4082
- Package Weight (in Pounds): 5
- Size: 1 x 96 Preps /4 x 96 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
