Quick-DNA Urine Kit
| Cat # | Name | Size |
|---|---|---|
| D3061 | Quick-DNA Urine Kit | 50 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- Purify cellular and/or cell-free DNA easily and reliably from up to 40 ml of urine.
- Uniquely formulated urine conditioning reagent allows stabilization of DNA in urine for up to 1 month at ambient temperature.
- Zymo-Spin column technology ensures DNA is ready for all sensitive downstream applications including qPCR, DNA sequencing, arrays, and DNA methylation analysis.
Description
The Quick-DNA Urine Kit is an innovative product designed for easy, reliable, and rapid isolation of cellular and/or cell-free DNA from up to 40 ml of urine. The product features a uniquely formulated urine DNA stabilization reagent that also functions as a precipitation reagent. After collection, total (cellular and cell-free) or cell-free urine can be stored at ambient temperature for up to one month by adding the Urine Conditioning Buffer. When ready to extract the urine DNA, just add the Clearing Beads, vortex, and centrifuge to collect the precipitate. Following precipitation, chemical lysis and enzymatic digestion are used to extract DNA from the precipitate. The DNA is subsequently purified and concentrated using Zymo-Spin IC-S Columns. Urine DNA isolated with the Quick-DNA Urine Kit is ideal for qPCR, array, methylation analysis, and other downstream applications.
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment
Centrifuge, microcentrifuge, and heat block/bath.
Processing Volume
≤ 40 ml
Purity
Typical A260/A280 ≥ 1.8
Sample Source
Urine
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
100 bp – 23 kb
Type
Total DNA including cfDNA
Yield
Up to 5 µg total DNA can be eluted into ≥ 10 µl. 6 – 1000 ng/ml for healthy females. 2 – 20 ng/ml for healthy males.
Q1: If I plan to process my urine samples immediately, is the Urine Conditioning Buffer necessary?
The Urine Conditioning Buffer (UCB) is necessary if you are interested in isolating cell-free DNA. If omitting the UCB, only cellular DNA is recovered.
Q2: What is the function of the Clearing Beads?
When the Urine Conditioning Buffer is added to the urine, the Clearing Beads helps to create a compact pellet and make it the pellet easy to visualize during the procedure.
Q3: Can I process urine samples stabilized in DNA/RNA Shield?
Yes, however, the volume of sample processed becomes large and difficult to handle. In addition, cellular and cell-free DNA cannot be separated. To process urine samples stabilized in DNA/RNA Shield, please contact technical support at tech@zymoresearch.com.
Q4: What should I do if I see an oil layer on top of the pellet, after centrifugation?
Remove the oil layer by pipetting and process the pellet. However, residual oil will not affect purification.
Q5: Can cell-free DNA be isolated from urine stored at – 80 °C?
We recommend stabilizing fresh urine with the Urine Conditioning Buffer and storing stabilized urine for later processing. Frozen urine can be processed, however, cells may lyse open upon freeze-thaw, causing in fragmentation of gDNA and resulting in more cell-free DNA than what was originally present.
Q6: Is the Quick-DNA Urine kit compatible with DNA Urine Preserve from Streck?
Yes, however, we cannot guarantee the efficiency of the Streck preservative.
DNA was extracted from urinary epithelial cells, non-invasively available tissue, using the Quick-DNATM Urine Kit in study of the Old Order Amish population in the US suspected of having mitochondrial DNA mutations. Collected urine samples were assessed for heteroplasmy level for the m.3243A>G mutation using high-resolution melt (HRM) profiling and confirmed through DNA Sanger sequencing. This finding represents the first report of mitochondrial respiratory chain disorders in the Amish community and suggests that it may be under-diagnosed.
Researches used the Quick-DNA Urine Kit (previously known as Extract-all Urine DNA kit) to detect and investigate the role of tumor cell-free DNA copy number variations (CNVs) in urine as clinically relevant diagnostic and predictive biomarkers for metastatic hormone sensitive and metastatic castrate resistant stages of prostate cancer. Urine cfDNAs derived from Quick-DNA Urine Kit were used to perform whole genome sequencing to generate high quality sequencing library. Researchers were able to detect CNVs in all samples, furthermore they were able to detect specific urine cfDNAs genomic aberrations at loci of PTEN, TMPRSS2, AR, and NOTCH1. CNV changes after treatment (androgen deprivation therapy and doxetacel chemotherapy) allowed for the identification of treatment-associated CNV changes, most important of which have been reported to be aberrant in prostate cancer such as copy number changes in RNF43 and ZNRF3 loci and in the LGR4 and MYC proto oncogene loci. These findings could prove potential predictive and prognostic classifiers for advance prostate cancer patients.
“Good yields of DNA in a very simple efficient process.”
– R. W. (Medical University of South Carolina)
“The kit is very useful as it lets us to analyze big volumes without an ultracentrifugation step.”
– S. B.M. (University of Barcelona)
“The protocol was easy to use/follow and it didn’t take a great deal of time to get results.”
– S. H. (South College School of Pharmacy)
“We tested it this week and it worked beautifully. We really would like to order this kit to process more samples!”
– R. D. (Medical College of Wisconsin)
“The Zymo Kit and more specifically the Cell-DNA method presents as the best option to improve the outcome of our LR-PCR method.”
– R. D. (University of Sydney and Royal North Shore Hospital)
“Urine DNA isolation kit is very much needed and I like Zymo kits for the simplicity an reliability.”
– A. C. (Sanford-Burnham Medical Research Institute)
| Cat # | Name | Size |
|---|---|---|
| D3004-4-4 | DNA Elution Buffer | 4 ml |
| D3004-1-50 | Genomic Lysis Buffer | 50 ml |
| D3061-1-140 | Urine Conditioning Buffer | 140 ml |
| D3061-2-1 | Clearing Beads | 1 ml |
| D3061-3-20 | Urine Pellet Digestion Buffer | 20 ml |
| D3061-4-10 | Urine DNA Prep Buffer | 10 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1015-50 | Zymo-Spin IC-S Columns | 50 Pack |
| D3061-5-12 | Urine DNA Wash Buffer | 12 ml |
