Quick-DNA Viral Kit
| Cat # | Name | Size |
|---|---|---|
| D3015 | Quick-DNA Viral Kit | 50 Preps |
| D3016 | Quick-DNA Viral Kit | 200 Preps |
Documents
Protocol:
Datasheet
SDS (MSDS):
Description
Highlights
- Quick recovery of viral DNA from a wide range of sources using Zymo-Spin column technology.
- Column design allows DNA to be eluted at high concentrations into minimal volumes.
- Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Description
The Quick-DNA Viral Kits are viral DNA purification kits that provide for the rapid isolation of high-quality viral DNA from a wide range of biological sources. A uniquely designed buffer is included for the efficient denaturation of viral particles in whole blood (fresh and stored), plasma, serum, tissue, ascites, cultured cells, and from liquid samples. DNA can be eluted with elution buffer or water and is suitable for subsequent PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation sequencing.
Equipment
Microcentrifuge, vortex
Processing Volume
≤ 200 µl Biological Fluids or ≤ 200 µl cells at a concentration of ≤ 105 cells/ml
Purity
Typical A260/A280 ≥ 1.8
Sample Source
Whole blood (fresh and stored), plasma, serum, tissue, ascites, cultured cells, and liquid samples.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
100 bp – 50 kb
Type
Total DNA
Yield
Up to 5 µg total DNA can be eluted into ≥ 6 µl
Q1: Does the kit separate genomic DNA from viral DNA?
No, the Quick-DNA Viral Kit will isolate total DNA from the sample including host genomic DNA and viral DNA.
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99 / 100 Bioz Stars |
Complete Genome Sequences of Escherichia coli Phages vB_EcoM-EP75 and vB…
Microbiol Resour Announc – Published 18 Apr 2019
‘After centrifugation, the supernatant was filter sterilized (0.2-µm membrane filter) and used in two consecutive rounds of plaque isolation to obtain a pure phage solution. .. Phage DNA was extracted using the Quick-DNA viral kit (Zymo Research, Irvine, CA) according to the manufacturer’s protocol.’
Peroxisomes support human herpesvirus 8 latency by stabilizing the viral…
PLoS Pathog – Published 10 May 2018
enzyme heat activation step of 10 min at 95°C, followed by 40 cycles of 15 seconds at 95°C for denaturing and 1 min at 60°C for annealing and extension. . For determination of encapsidated HHV-8 genome copy number from the culture supernatant, viral DNA was purified using Quick-DNA™ Viral Kit (Zymo Research) following pretreatment of virus suspension with DNase I (New England BioLabs) at 37°C overnight.. RT-qPCR was performed using ABI PRISM 7500 system as described above with the LANA primers ( )
Distribution of Porcine Cytomegalovirus in Infected Donor Pigs and in Ba…
Viruses – Published 06 Feb 2018
‘Briefly, DNA was extracted from sera and blood of the pigs using two DNA extraction kits, a DNeasy Blood and Tissue kit (Qiagen GmbH, Hilden, Germany) and a ZR viral DNA kit (Zymo Research Corp., Irvine, CA, USA).’
| Cat # | Name | Size |
|---|---|---|
| D3004-4-10 | DNA Elution Buffer | 10 ml |
| D3004-4-4 | DNA Elution Buffer | 4 ml |
| D3015-1-50 | ZR Viral DNA Buffer | 50 ml |
| D3016-1-100 | ZR Viral DNA Buffer | 100 ml |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
