Quick-RNA FFPE Miniprep
| Cat # | Name | Size |
|---|---|---|
| R1008 | Quick-RNA FFPE Miniprep | 50 preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
R1008
Highlights
Easy Processing: Included Deparaffinization Solution for simple paraffin removal. No xylene necessary.
Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery.
High Quality: Non-crosslinked DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
Description
The Quick-RNA FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of the product have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.
Equipment
Microcentrifuge, vortex, heat block/bath
Purity
RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source
Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
Size Range
Total RNA ≥ 17 nt
Yield
50 µg RNA (binding capacity), ≥25 µl (elution volume)
| Cat # | Name | Size |
|---|---|---|
| W1001-10 | DNase/RNase-Free Water | 10 ml |
| D3067-1-20 | Deparaffinization Solution | 20 ml |
| R1003-3-24 | RNA Wash Buffer | 24 ml |
| R1060-1-50 | RNA Lysis Buffer | 50 ml |
| R1060-2-25 | RNA Prep Buffer | 25 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| E1010-1-4 | DNA Digestion Buffer | 4 mL |
| D3050-1-5 | 2X Digestion Buffer – 5 mL | 5 mL |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack |
