Quick-RNA Fungal/Bacterial Microprep Kit
| Cat # | Name | Size |
|---|---|---|
| R2010 | Quick-RNA Fungal/Bacterial Microprep Kit | 50 preps |
Documents
Protocol:
SDS (MSDS):
Description
R2010
Highlights
High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
Description
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
Applicable For
For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Elution Volume
≥ 6 µl
Equipment
Microcentrifuge, vortex, cell disrupter/pulverizer
Processing Time
≤ 10 minutes
Processing Volume
10-20 mg (wet weight) fungi or bacteria, 2×108 bacterial cells, or 2×107 yeast cells
Purity
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8.
Sample Source
Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
Size Range
Total RNA ≥ 17 nt
Type
Total RNA
Yield
Microprep
10 µg RNA (binding capacity)
≥ 6 µl (elution volume)
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99 / 100 Bioz Stars |
Paenibacillus amylolyticus 27C64 has a diverse set of carbohydrate-activ…
J Ind Microbiol Biotechnol Published 30 Oct 2019
‘CMC was purchased from Sigma (St. Louis, MO).. Total RNA was isolated from cells grown in TSB without pectin and in TSB supplemented with 0.4% w/v of either PGA, apple pectin, or RG-I from soy at 12.75 h after inoculation with the ZR Fungal/Bacterial RNA Miniprep kit (Zymo Research, Irvine, CA).. Ten µg of RNA was treated with DNase using the TURBO DNA-free Kit (Thermo Fisher, Waltham, MA).’
Selection Pressure Pathways and Mechanisms of Resistance to the Demethyl…
Front Microbiol Published 31 Oct 2018
‘of the gene were concatenated and a multiple alignment was constructed using BioEdit Version 7.2.5 ( ) to determine nucleotide and amino acid changes. . Total RNA was isolated from DIF-free 14-day-old PDA plates using a ZR Fungal/Bacterial RNA MiniPrep Kit (Zymo Research, Irvine, CA, United States) according to the supplier’s instructions.. All RNA was analyzed for quantity and quality spectroscopically on 1% TBE agarose gel.’
Phylogenetic analysis of West Nile Virus in Maricopa County, Arizona: Ev…
PLoS One – Published 26 Nov 2018
(2017), for whole genome tiled amplicon sequencing using a novel protocol, based on the method of Quick et al. developed for Zika virus sequencing. . Prior to transporting the samples, an equal part of DNA/RNA ShieldTM 2X Concentrate (Zymo Research) was added to each mosquito pool to stabilize the RNA and inactivate WNV.. The pools consisted of mosquitos in approximately 1.3 ml of TE buffer (Invitrogen, AM9858).
