Quick-RNA Fungal/Bacterial Miniprep Kit
| Cat # | Name | Size |
| R2014 | Quick-RNA Fungal/Bacterial Miniprep Kit | 50 preps |
Documents
Protocol
Data Sheet
SDS (MSDS)
Description
R2014
Highlights
High Quality: Extract intact high quality total RNA (including small/micro RNAs) from Gram-negative/postive bacteria, yeast, and fungi
Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
Description
The Quick-RNA Fungal/Bacterial Microprep Kit is designed for the simple and rapid isolation of RNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the RNA that is ideal for downstream molecular-based applications including Next-Gen sequencing, RT-PCR, microarray, hybridization, etc.
Applicable For
For all sensitive downstream applications, such as Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Elution Volume
25 µl
Processing Time
≤ 10 minutes
Processing Volume
50-100 mg (wet weight) fungi or bacteria. 109 bacterial cells and 108 yeast cells
Purity
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc.
A260/A280, A260/A230: > 1.8.
Size Range
Total RNA ≥ 17 nt
Type
Total RNA
Yield
Miniprep
50 µg RNA (binding capacity)
≥ 25 µl (elution volume)
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99 / 100 Bioz Stars |
Paenibacillus amylolyticus 27C64 has a diverse set of carbohydrate-activ…
J Ind Microbiol Biotechnol Published 30 Oct 2019
‘CMC was purchased from Sigma (St. Louis, MO).. Total RNA was isolated from cells grown in TSB without pectin and in TSB supplemented with 0.4% w/v of either PGA, apple pectin, or RG-I from soy at 12.75 h after inoculation with the ZR Fungal/Bacterial RNA Miniprep kit (Zymo Research, Irvine, CA).. Ten µg of RNA was treated with DNase using the TURBO DNA-free Kit (Thermo Fisher, Waltham, MA).’
Selection Pressure Pathways and Mechanisms of Resistance to the Demethyl….
Front Microbiol Published 31 Oct 2018
‘of the gene were concatenated and a multiple alignment was constructed using BioEdit Version 7.2.5 ( ) to determine nucleotide and amino acid changes. . Total RNA was isolated from DIF-free 14-day-old PDA plates using a ZR Fungal/Bacterial RNA MiniPrep Kit (Zymo Research, Irvine, CA, United States) according to the supplier’s instructions.. All RNA was analyzed for quantity and quality spectroscopically on 1% TBE agarose gel.’
Low-cost detection of norovirus using paper-based cell-free systems and …
Synth Biol Oxf Published 19 Sep 2018
‘700926), methicillin-resistant Staphylococcus aureus MRSA252 (ATCC, BAA-1720) and Bacillus subtilis 168 (ATCC, 23857) were used for assay cross-reactivity experiments. . For these experiments, RNA from the bacteria was extracted using a Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research) following the manufacturer’s instructions.. To obtain purified viral RNA for cross-reactivity experiments, 5 µl of GII.4, GI.2 and GI.6 positive stool samples were suspended in 140 µl RNase-free ‘
