Quick-RNA Miniprep Plus Kit
| Cat # | Name | Size: |
|---|---|---|
| R1057T | Quick-RNA Miniprep Plus Kit | 10 preps |
| R1057 | Quick-RNA Miniprep Plus Kit | 50 preps |
| R1058 | Quick-RNA Miniprep Plus Kit | 200 preps |
Documents
Protocol:
SDS (MSDS): R1057/R1058
SDS (MSDS): R1057T
Description
R1057T / R1057 / R1058
Highlights
Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
DNA-Free: Genomic DNA removal column and DNase I included.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Description
The Quick-RNA Miniprep Plus Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from cells, all tissue types, whole blood, and biological fluids. The provided DNA/RNA Shield stabilizes samples, allowing them to be stored without the need for immediate freezing or processing for up to one month. Furthermore, DNA/RNA Shield™ inactivates RNases as well as microbial pathogens (viruses, bacteria, etc.). The procedure combines a unique buffer system with Zymo-Spin Column technology to yield high quality total RNA (including small RNAs 17-200 nt). Simply add DNA/RNA Shield and Proteinase K to extract total RNA from any tissue, then purify the RNA using the Zymo-Spin Column. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing, etc. In addition, the kit can be used for the enrichment of small and large RNAs in two separate fractions.
Equipment
| Microcentrifuge, vortex, heat block/bath (55ºC) |
Purity
| RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
Sample Source
| Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and enzymatic reactions (e.g., DNase I), samples in DNA/RNA Shield™, TRIzol® or RNAlater™. |
Size Range
| Total RNA ≥ 17 nt |
Yield
| spin column, 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q4: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q5: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q6: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q7: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q9: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.
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99 / 100 Bioz Stars |
Targeting p53-dependent stem cell loss for intestinal chemoprotection
Sci Transl Med Published 07 Feb 2019
‘Fresh mucosal scrapings from 10 cm of jejunum were washed in cold PBS, resuspended in 700 μl of RNA lysis buffer, and homogenized in a Dounce homogenizer. . RNA was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions.. Organoids were freed from Matrigel using Cell Recovery Solution (Corning), as described in , before RNA isolation.’
Cellular acidosis triggers human MondoA transcriptional activity by driv….
Nat Commun – Published 21 Dec 2018
‘A list of plasmids used, the vector backbone and their source is provided in the Key Resources Table.. Total cellular RNA was extracted using a Quick RNA Miniprep Kit (Zymo Research) according to manufacturer’s recommendations. cDNA was synthesized from 200 ng mRNA using the GoScript Reverse Transcription System (Promega) with oligo-dT primers.. A 100-fold dilution was used in a PCR reaction containing SYBR Green and analyzed on a CFX Connect Real Time System.’
Promotion of Myoblast Differentiation by Fkbp5 via Cdk4 Isomerization
Cell Rep Published 29 Jan 2019
‘Secondary antibodies were diluted in 5% milk in TBST and six subsequent washes were done with TBST.. RNA was extracted from cells using a Quick RNA Microprep (Zymo Research, R1051) or RNeasy Plus Mini (QIAGEN, 74136) kit, depending on cell number.. RNA quantity and purity were assessed using a NanoDrop Lite spectrophotometer (Thermo Fisher Scientific). cDNA was synthesized with ProtoScript II ‘
“RNA isolation from tissue culture cells used to be my most hated protocol – labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit.”
– Lisa G. (University of Virginia)
“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”
– Erin B. (Occidental College)
“Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit.”
– Xiaolu J. (Florida Atlantic University)
Kit Components
| Cat # | Name | Size | ||
|---|---|---|---|---|
| R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml | ||
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | ||
| W1001-1 | DNase/RNase-Free Water | 1 ml | ||
| W1001-30 | DNase/RNase-Free Water | 30 ml | ||
| W1001-6 | DNase/RNase-Free Water | 6 ml | ||
| R1003-3-24 | RNA Wash Buffer | 24 ml | ||
| R1003-3-48 | RNA Wash Buffer | 48 ml | ||
| R1060-1-100 | RNA Lysis Buffer | 100 ml | ||
| R1060-1-50 | RNA Lysis Buffer | 50 ml | ||
| R1060-2-100 | RNA Prep Buffer | 100 ml | ||
| R1060-2-25 | RNA Prep Buffer | 25 ml | ||
| R1200-1-20 | PK Digestion Buffer | 20 ml | ||
| R1200-1-5 | PK Digestion Buffer | 5 ml | ||
| C1001-50 | Collection Tubes | 50 Pack | ||
| C1006-50-F | Spin-Away Filters | 50 Pack | ||
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | ||
| E1010-1-4 | DNA Digestion Buffer | 4 mL | ||
| E1010-1-16 | DNA Digestion Buffer | 16 mL | ||
