Quick-RNA Tissue/Insect Kit

Quick-RNA Tissue/Insect Kit

Cat # Name Size
R2030 Quick-RNA Tissue/Insect Microprep Kit 50 preps

Documents

Protocol

Data Sheet

SDS (MSDS)

Categories: , ,

Description

R2030

Highlights

High Quality: Extract intact high quality total RNA (including small/micro RNAs) from insect and arthropod specimens (mosquitoes, bees, lice, ticks, Drosophila melanogaster etc.)

Homogenization: Ultra-high density BashingBead technology for complete disruption of tough specimen.

Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.

Description

The Quick-RNA Tissue/Insect Microprep Kit provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). The Quick-RNA Tissue/Insect Microprep Kit employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.

Equipment

Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)

Purity

RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.

Sample Source

Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster)

Size Range

Total RNA ≥ 17 nt

Yield

10 µg RNA (binding capacity), ≥6 µl (elution volume)

Kit Components

Cat # Name Size
W1001-1 DNase/RNase-Free Water 1 ml
R1003-3-12 RNA Wash Buffer 12 ml
R1060-1-50 RNA Lysis Buffer 50 ml
R1060-2-25 RNA Prep Buffer 25 ml
C1001-50 Collection Tubes 50 Pack
C1004-50 Zymo-Spin IC Columns 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
S6003-50 ZR BashingBead Lysis Tubes (2 mm) 50 Tubes