Quick-RNA Viral Kit
| Cat # | Name | Size |
| R1034 | Quick-RNA Viral Kit | 50 preps |
| R1035 | Quick-RNA Viral Kit | 200 preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
R1034 / R1035
Highlights
Viral RNA: Compatible with plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies, swabs, feces, samples in DNA/RNA Shield, etc.
Streamlined Workflow: Sample inactivation and easy one-step lysis enables fast processing.
High Sensitivity: Optimized for low viral copy detection for Next-Gen Sequencing and RT-qPCR.
Description
The Quick-RNA Viral Kit is a quick viral RNA purification kit for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™. DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures (4-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit also features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the column, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen Sequencing, hybridization-based and RT/PCR detection.
Equipment
Microcentrifuge
Purity
RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Sample Source
Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, and swab and fecal samples stored in DNA/RNA Shield
Size Range
50 nt to ~200 kb
Yield
10 µg RNA (binding capacity), ≥6 µl (elution volume)
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98 / 100 Bioz Stars |
Norovirus Cell Tropism is Determined by Combinatorial Action of a Viral …
Cell Host Microbe Published 11 Oct 2018
‘Plates were incubated for 60–72 hours prior to visualization of plaques with crystal violet solution (0.2% crystal violet and 20% ethanol).. As previously described , RNA was isolated from stool using a ZR-96 viral RNA kit (Zymo Research, Irvine, CA).. RNA from tissues or cells was isolated using TRI Reagent (Invitrogen) with a Direct-zol-96 RNA kit (Zymo Research, Irvine, CA) according to the manufacturer’s ‘
Viral Entry Properties Required for Fitness in Humans Are Lost through R…
‘mNGS was performed as previously described ( ).. RNA was extracted from 100 µl of thawed culture harvests and clinical samples using the Zymo Viral RNA kit (Zymo Research) and treated with Turbo DNase I (Thermo Fisher) for 30 min at 37°C ( ).. Double-stranded cDNA was made using random hexamers, SuperScript III reverse transcriptase (Thermo Fisher), and Sequenase v2.0 (Thermo Fisher) and ‘
Serosurvey of Human Antibodies Recognizing Aedes aegypti D7 Salivary Pro…
Front Public Health Published 18 May 2018
‘RNA extracted from serum samples according to methods described by Londono-Renteria et al. ( ) using DENV serotypes specific primers described elsewhere ( ). . Briefly, RNA was extracted from the participant’s serum sample using the Quick RNA-Viral kit (Zymo Research). qRT-PCR conditions on the CFX96 Touch™ Real-Time PCR Detection System were: RT Step: 48°C for 5 min, and 95°C for 2 min. Amplification step: (95°C for 15 s, 60°C for 20 s) × 40 cycles.. The living conditions of participants are based on the socioeconomic stratification for the Barrios (Neighborhoods) in the city (Cucuta) according ‘
“The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”
– S.B., Boehringer Ingelheim, Canada
