Quick RNA Viral Kit – DX
| Cat# | Name | Size |
|---|---|---|
| R1034-E | Quick RNA Viral Kit - DX | 50 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS)
Description
Highlights
- Viral RNA: Compatible with plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies, swabs, feces, samples in DNA/RNA Shield, etc.
- Streamlined Workflow: Sample inactivation and easy one-step lysis enables fast processing.
- High Sensitivity: Optimized for low viral copy detection for Next-Gen Sequencing and RT-qPCR.
Description
The Quick-RNA Viral Kit is a quick, purification system for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™. DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures (4-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit also features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the column, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen Sequencing, hybridization-based and RT/PCR detection.
Equipment
Microcentrifuge
Purity
RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Registration Status
CE-IVD certified (R1034-E, R1035-E)
Sample Source
Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, and swab and fecal samples stored in DNA/RNA Shield
Yield
10 µg RNA (binding capacity), ≥6 µl (elution volume)
Q1: Is the addition of beta-mercaptoethanol necessary?
The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.
Q2: Is this kit compatible with samples stored in UTM/VTM?
Yes, these samples are compatible.
Q3: Will this kit isolate DNA?
Yes, this kit will co-purify some DNA.
Q4: Can DNase I treatment be performed?
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Q5: Is it normal for the Viral RNA (DNA/RNA) Buffer to range in color between clear and yellow?
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
Q6: What is the expiration of the Viral RNA Buffer when beta-mercaptoethanol has been added?
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
Q7: Why are my columns/plate wells getting clogged?
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
Q8: Why am I seeing delayed/no amplification during RT/qPCR?
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
CITATIONS
| Cat# | Name | Size |
|---|---|---|
| R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml |
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml |
| W1001-10 | DNase/RNase-Free Water | 10 ml |
| W1001-4 | DNase/RNase-Free Water | 4 ml |
| R1034-1-100 | Viral RNA Buffer | 100 ml |
| R1034-1-50 | Viral RNA Buffer | 50 ml |
| R1034-2-48 | Viral Wash Buffer (concentrate) | 48 ml |
| R1034-2-6 | Viral Wash Buffer (concentrate) | 6 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
