Quick-RNA Whole Blood

Quick-RNA Whole Blood

Cat # Name Size
R1201 Quick-RNA Whole Blood 50 preps

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Description

R1201

Highlights

Superior Yields: Recover total RNA (including small/micro RNAs) without sample loss.

Protection: Worry-free blood sample storage at ambient temperatures for up to 30 days.

High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc. DNase I Included.

Description

The Quick-RNA Whole Blood kit utilizes DNA/RNA Shield™, a unique preservation and lysis technology, to enable rapid isolation of total RNA from whole or partitioned blood or a cell pellet (after red blood cell lysis). The procedure uses Zymo-Spin column technology in which the sample is pre-filtered on the Zymo-Spin IIICG Column and then purified and concentrated on the Zymo-Spin IC Column. RNA is eluted into ≥ 6 µl of RNase-free water and is ready for any downstream application including RT-PCR, sequencing, etc.

Equipment

Microcentrifuge, vortex

Purity

RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.

Sample Source

Up to 1 ml mammalian whole-blood, plasma, or serum. Also compatible with pelleted blood cells (PBMCs, WBCs, buffy coat, pelleted sample from PAXgene™ Blood RNA Tube, etc.) and nucleated blood.

Size Range

Total RNA ≥ 17 nt

Yield

10 µg RNA (binding capacity), ≥6 µl (elution volume)

99 / 100
Bioz Stars

RNA was extracted from canine blood using the Quick-RNA Whole Blood kit and used for PCR amplification for cloning canine TREM-1. TREM-1 expression was shown to be an amplifier or pro-inflammatory responses and has potential to be a biomarker for infection and pneumonia.
Li, J et al. Expression and function of triggering receptor expressed on myeloid cells-1 (TREM-1) on canine neutrophils. Developmental and Comparative Immunology. 2011.

The Quick-RNA Whole Blood kit was used to purify 200 ul human blood and RNA was used to generate cDNA for cloning. Data suggests that the SNPs of aminopeptidase ERAP1 can encode for different normal, hypo-, or hyper- functional activities.

Reeves, E et al. Naturally Occurring ERAP1 Haplotypes Encode Functionally Distinct Alleles with Fine Substrate Specificity. The Journal of Immunology. 2013.

HCV RNA was extracted from sera of human patients using the Quick-RNA Whole Blood kit and used for amplification and Roche 454 pyrosequencing. Results reveal that more genotype 1a isolates were associated with resistance to protease inhibitors.

Margeridon-Thermet, S et al. Similar Prevalence of Low-Abundance Drug-Resistant Variants in Treatment-Naïve Patients with Genotype 1a and 1b Hepatitis C Virus Infections as Determined by Ultradeep Pyrosequencing. PLoS ONE. 2014

The Quick-RNA Whole Blood kit was used with peripheral blood stored at 4°C and extracted total RNA was used for real-time PCR gene expression analysis. Results suggest that upregulation of mRNA levels of ATP13A1, PARK, and ZNF746 can be observed in both untreated patients and preclinical stages of Parkinson’s disease.

Alieva, AK et al. Potential Biomarkers of the Earliest Clinical Stages of Parkinson’s Disease. Parkinson’s Disease. 2015.

Researchers used the Quick-RNA Whole Blood kit to extract total RNA from blood cell pellets derived from bovine blood stored in anticoagulant ACD. High RNA integrity (RIN >7) was obtained and used for microarray hybridization.

Adjei-Fremah, S et al. Transcriptional profiling of the effect of lipopolysaccharide (LPS) pretreatment in blood from probiotics-treated dairy cows. Genomics Data. 2016.

The Quick-RNA Whole Blood kit was used to extract RNA from human blood stored in DNA/RNA Shield Blood Collection Tubes and then Minichromosome maintenance complex component (MCM) 8 and 9 expression were assessed by qPCR. Greater MCM8 expression occurred in the follicular phase of the menstrual cycle.

Dondik, Y et al. Minichromosome maintenance complex component 8 and 9 gene expression in the menstrual cycle and unexplained primary ovarian insufficiency. Journal of Assisted Reproduction and Genetics. 2019.

Kit Components

Cat # Name Size
R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml
W1001-4 DNase/RNase-Free Water 4 ml
R1003-3-24 RNA Wash Buffer 24 ml
R1060-2-25 RNA Prep Buffer 25 ml
R1070-1-10 RNA Recovery Buffer 10 ml
R1200-1-5 PK Digestion Buffer 5 ml
C1001-50 Collection Tubes 50 Pack
C1004-50 Zymo-Spin IC Columns 50 Pack
C1006-50-G Zymo-Spin IIICG Columns 50 Pack
E1010-1-4 DNA Digestion Buffer 4 mL