RNA Clean & Concentrator-25
| Cat # | Name | Size |
| R1017 | RNA Clean & Concentrator-25 | 50 |
| R1018 | RNA Clean & Concentrator-25 | 100 |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Maximum Recovery: Recover >90% and elute in as little as 25 μl.
Description
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 25 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Equipment
Microcentrifuge
Format
Spin-Column
Purity
RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source
DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size Range
Total RNA ≥ 17 nt
Yield
50 µg RNA (binding capacity), ≥ 25 µl (elution volume)
Q1: Can the RCC kit be used to purify DNA (cDNA)?
Yes. The kit efficiently recovers all types of nucleic acids.
Q2: Should carrier RNA be added to the sample prior to purification?
Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.
Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?
Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.
Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.
Q6: Is DNase I treatment necessary?
If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.
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99 / 100 Bioz Stars |
Breaching self-tolerance to Alu duplex RNA underlies MDA5-mediated infla……
Cell Published 08 Feb 2019
The final RNA was used for RT-qPCR-based quantitation.. RNAs recovered from the RNase A protection assay (cytoRNA-0.0 and −2.0) were treated with DNase I (New England Biolabs) to remove any DNA contaminations and concentrated by RNA clean & concentrator kit (Zymo Research).. Following depletion of rRNA using Next rRNA-depletion kit (New England Biolabs), 100 ng RNA was used for the cDNA library construction using Smarter ‘ (Less…)
“Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant.”
– Catherine A. (USAMRIID)
“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
– Caroline S. (Cornell University)
“The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns.”
– Agnes N. (Biofidal)
“Zymo RNA Clean & Concentrator is the best product for CRISPR applications. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield.”
– Gaurav V. (OMRF)
“Great result. Everybody should try this product. I’ve never seen such purity of the RNA before. This product works excellent and easy to use and affordable.”
– Para S. (University of Louisville)
“I used this as a clean-up step after guanidinium thiocyanate-phenol-chloroform extraction from homogenized tissues. I found the process very simple, and adding the kit on did seem to significantly improve the quality of the RNA pool relative to the original phenol-chloroform extraction.”
– Emily G. (University of Wisconsin-Madison)
