RNA Clean & Concentrator-96
| Cat # | Name | Size |
| R1080 | RNA Clean & Concentrator-96 | 2 x 96 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
R1080
Highlights
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Maximum Recovery: Recover > 90% and elute in as little as 6 µl.
Description
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Equipment
| Centrifuge with microplate carriers |
Format
| Spin-Column |
Purity
RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source
| DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
Yield
| 25 µg RNA (binding capacity), ≥ 10 µl (elution volume) |
Size Range
| Total RNA ≥ 17 nt |
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99 / 100 Bioz Stars |
Breaching self-tolerance to Alu duplex RNA underlies MDA5-mediated infla…
‘The final RNA was used for RT-qPCR-based quantitation.. RNAs recovered from the RNase A protection assay (cytoRNA-0.0 and −2.0) were treated with DNase I (New England Biolabs) to remove any DNA contaminations and concentrated by RNA clean & concentrator kit (Zymo Research).. Following depletion of rRNA using Next rRNA-depletion kit (New England Biolabs), 100 ng RNA was used for the cDNA library construction using Smarter ‘
Feed Restriction Modifies Intestinal Microbiota-Host Mucosal Networking …
mSystems Published 29 Jan 2019
‘high-RFI chickens ( ) using mechanical homogenization (FastPrep-24 instrument; MP Biomedicals, Santa Ana, CA) and the RNeasy Mini kit (Qiagen, Hilden, Germany). . The RNA isolates were treated with DNase I (RNA Clean & Concentrator-5 kit, Zymo Research, Irvine, USA) before transcribing 2 µg of total RNA into single stranded cDNA using the High Capacity Reverse Transcription kit (Life Technologies Foster City, USA).. The quality (q) of the isolated RNA was verified using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA), showing RNA integrity ‘
““Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
– Caroline S. (Cornell University)
“The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns.”
-Agnes N. (Biofidal)
“Zymo RNA Clean & Concentrator is the best product for CRISPR applications. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield.”
-Gaurav V. (OMRF)
| Cat # | Name | Size |
|---|---|---|
| W1001-6 | DNase/RNase-Free Water | 6 ml |
| R1003-3-24 | RNA Wash Buffer | 24 ml |
| R1013-2-100 | RNA Binding Buffer | 100 ml |
| R1060-2-25 | RNA Prep Buffer | 25 ml |
| C2002 | Collection Plate | 2 Plates |
| C2003 | Elution Plate | 2 Plates |
| C2004 | Zymo-Spin I-96 Plate | 2 Plates |
| C2007-4 | 96-Well Plate Cover Foil | 4 Foils |
