Yeast Protein Kit
| Cat# | Name | Size |
|---|---|---|
| Y1002 | Yeast Protein Kit | 200 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- Convenient: Rapid method for efficient lysis of yeast for downstream protein and DNA analyses.
- Versatile: Procedure suitable for any fungal species susceptible to Zymolyase.
- Effective Spheroplasting: Ideal protocol for Western blotting and PCR.
Description
The Yeast Protein Kit is a simple and convenient method for the rapid and thorough lysis of yeast cells. The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme digestion. The digestion procedure effectively generates spheroplasts of yeast cells, making them ideal for both protein and DNA analyses including Western blotting and PCR, respectively.
Applicable For
Spheroplasts are ideal for both protein and DNA analyses including Western blotting and PCR, respectively.
Equipment
Microcentrifuge & Heat Block/Bath
Device Storage
Ambient temperature => 2 years
Processing Time
35 Minutes
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Q1: What strains is this kit compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis
| Cat # | Name | Size |
|---|---|---|
| Y1002-1-6 | Y-Lysis Buffer | 6 ml |
- Catalog#: Y1002
- Package Length (in Inches): 5.3
- Package Width (in Inches): 3.3
- Package Height (in Inches): 0.5
- Package Weight (in Pounds): 0.1
- Size: 200 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
