RT-PCR was used to analyze S1 nuclease protection using RNA extracted from Saccharomyces cerevisiae strains with the YeaStar RNA kit. Researchers showed that histone H2B monoubiquitylation and it deubiquitylation are related in gene activation.

Henry, KW et al. Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes & Development. 2003.

The YeaStar RNA kit was used to extract total RNA from Saccharomyces cerevisiae cells and spheroblasts for downstream qRT-PCR quantification. The WEE1 kinase was found to be an epigenetic modulator that inhibits transcription of histone genes.

Mahajan, K et al. H2B Tyr37 phosphorylation suppresses expression of replication-dependent core histone genes. Nature Structural & Molecular Biology. 2012.

Researchers found that two positive feedback loops regulated by Gal1p and Gal3p affect network activity and induce bimodal distribution of gene expression. The YeaStar RNA kit was used for yeast RNA extraction for real-time qPCR.

Venturelli, OS et al. Synergistic dual positive feedback loops established by molecular sequestration generate robus bimodal response. PNAS. 2012.

Abundance of heterologous mRNA was analyzed by quantitative RT-PCR using total RNA extracted from mid-log phase cells using the YeaStar RNA kit. mRNA half-life changes were determined to be the major cause of varied protein and transcript expression level.

Curran, KA et al. Use of expression-enhancing terminators in Sacchromyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications. Metabolic Engineering. 2013.

Transcriptional analysis of phenylalanine over-producing yeast mutants was performed using cDNA synthesized from RNA extracted with the YeaStar RNA kit. Saccharomyces cerevisiae was used a host of renewable styrene production, reaching titers of 29 mg/L and a 60% improvement over initial strains.

McKenna, R et al. Rational and combinatorial approaches to engineering styrene production by Saccharomyces cerevisiae. Microbial cell Factories. 2014.

Total RNA was extracted from Candid albicans using the YeaStar RNA kit for downstream qRT-PCR expression analysis of ZWF1, which is induced as a core stress response and involved in adaptive antioxidant response. Monoterpene phenols thymol and carvacrol was found to induce oxidative stress at lower concentrations.

Khan, A et al. Effect of two monoterpene phenols on antioxidant defense system in Candida albicans. Microbial Pathogenesis. 2015.

Different Komagataella strains comprising Pichia grown in YPD were used as sample input for the YeaStar RNA kit for long-read and short-read genome sequencing.

Love, KR et al. Comparative genomics and transcriptomics of Pichia pastoris. BMC Genomics. 2016.

The YeaStar RNA kit was used to extract RNA from Yarrowia liplytica cultures for RT-qPCR. Researchers were able to develop a CRISPR interference system for gene repression, achieving homologous recombination efficiencies as high as 90%.

Schwartz, C et al. CRISPRi repression of nonhomologous end-joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica. Biotechnology and Bioengineering. 2017.

Yeast RNA was extracted with the YeaStar RNA kit to test for CRISPR-Cas9 editing effects on gene expression using RT-qPCR. 572 yeast variants were identified with significant fitness differences, being enriched in promoters.

Sharon, E et al. Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing. Cell. 2018.

Total RNA was extracted from transformed yeast using the YeaStar RNA kit and passed RNA integrity assessment. Researchers analyzed the antiviral enzyme OAS1 functionality in yeast and found that loss-of-function alleles are common among non-human primates.

Carey, CM et al. Recurrent Loss-of-Function Mutations Reveal Costs to OAS1 Antiviral Activity in Primates. Cell Hose & Microbe. 2019.

See more details on Bioz