ZR Plasmid Miniprep – Classic
| Cat# | Name | Size |
|---|---|---|
| D4054 | ZR Plasmid Miniprep - Classic | 800 Preps |
| D4015 | ZR Plasmid Miniprep - Classic | 100 Preps |
| D4016 | ZR Plasmid Miniprep - Classic | 400 Preps |
Documents
Protocol:
SDS (MSDS):
Description
Highlights
- High Purity: Eluted plasmid DNA is high quality, endotoxin-free and ready for all sensitive downstream applications including transfections and sequencing.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization.
- Unique column design: Zero buffer retention and low (30 µl) elution volume.
Description
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin llN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Applicable For
Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, transfections, and other sensitive applications.
Elution Volume
≥ 30 µl
Endotoxin Levels
< 50 EU/µg
Equipment
Microcentrifuge
Processing Time
15 min
Purity
Typical Abs260/280 ≥1.8.
Size Range
Up to 25 kb
Yield
Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
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99 / 100 Bioz Stars |
Reprograming the replisome of a semi-synthetic organism for the expansio…
J Am Chem Soc – Published 17 Jan 2019
Cells were pelleted, decanted, and frozen after reaching an OD600 of 0.6–0.9.. In vivo replicated pINFs were isolated using a ZR Plasmid Miniprep-Classic kit according to manufacturer recommendations and advanced to biotin-shift PCR analysis (see ).. It should be noted that the Pol II+ Δ recA strain used in these experiments ( ) had a neo cassette at the former recA locus (P_polB(−)lexA-polB+FRT+ΔrecA+KanR+lacZYA
CRISPR/Cas9 and glycomics tools for Toxoplasma glycobiology
J Biol Chem – Published 21 Nov 2018
lasmid 1 REV. pDG plasmids were constructed by transferring the XhoI-NsiI fragment of p2 into p3 that was double-digested with XhoI and NsiI ( ). . Plasmids were prepared in transformed Top10 cells (Thermo Fisher Scientific) using a ZR Plasmid Miniprep-Classic (Zymo Research, D4016) kit.. Finally, the inserted guide sequences were confirmed by sequencing the pDG plasmid with primers gRNA For and gRNA Rev ( ).
Reprograming the replisome of a semi-synthetic organism for the expansio…
J Am Chem Soc – Published 17 Jan 2019
Cells were pelleted, decanted, and frozen after reaching 0.6–0.92 OD600 .. In vivo replicated pINFs were isolated using a ZR Plasmid Miniprep-Classic kit (Zymo Research) and a 5-μg silica column (Cat. #D4003, Zymo Research) according to manufacturer recommendations and advanced to biotin-shift PCR analysis (see ).. This procedure was preformed in at least triplicate for each knockout strain starting from preparation of electrocompetent cells.
| Cat # | Name | Size |
|---|---|---|
| D3004-4-16 | DNA Elution Buffer | 16 ml |
| D3004-4-4 | DNA Elution Buffer | 4 ml |
| D4027-1-160 | Buffer P1 (Red) | 160 ml |
| D4027-1-20 | Buffer P1 (Red) | 20 ml |
| D4027-1-80 | Buffer P1 (Red) | 80 ml |
| D4027-2-160 | Buffer P2 (Green) | 160 ml |
| D4027-2-20 | Buffer P2 (Green) | 20 ml |
| D4027-2-80 | Buffer P2 (Green) | 80 ml |
| D4027-3-220 | Buffer P3 (Yellow) | 220 ml |
| D4027-3-50 | Buffer P3 (Yellow) | 50 ml |
| D4027-4-24 | Plasmid Wash Buffer | 24 ml |
| D4027-4-48 | Plasmid Wash Buffer | 48 ml |
| D4027-4-6 | Plasmid Wash Buffer | 6 ml |
| D4036-3-30 | Endo-Wash Buffer | 30 ml |
| D4036-3-60 | Endo-Wash Buffer | 60 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| C1019-50 | Zymo-Spin IIN Columns | 50 Pack |
- Catalog#: D4054 /D4015 /D4016
- Package Length (in Inches): 12 /9/13
- Package Width (in Inches): 10.5 /6.5 /8
- Package Height (in Inches): .8 /6 /4.5
- Package Weight (in Pounds): 0.75 /6.9 /4.2
- Size: 800 Preps /100 Preps /400 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
