ZR small-RNA PAGE Recovery Kit
| Cat # | Name | Size |
| 1070 | ZR small-RNA PAGE Recovery Kit | 20 Preps |
Documents
Protocol:
SDS (MSDS):
Description
R1070
Highlights
- Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- Compatibility: Compatible with up to 25% (w/v) polyacrylamide.
Description
The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the “crush and soak” method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
Equipment
Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer
Purity
RNA is ready for all subsequent analysis and molecular manipulation
Sample Source
RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).
Size Range
RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)
Yield
10 µg RNA (binding capacity), ≥6 µl (elution volume)
Q1: What is the recovery rate?
The recovery rate for fragments 17 to 28 nucleotides is ≥50 %.
Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?
Yes, the kit is compatible with both single- or double-stranded RNA (and DNA) fragments.
Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?
Yes. Most customer’s do not use PAGE gels for to visualize fragments > 200 nucleotides, but it will be compatible with the kit.
Q4: Is the kit compatible with TBE-Urea PAGE?
Yes, this kit is compatible with TBE-Urea PAGE gels.
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q5: What is the maximum amount of gel that can be processed per prep?
There is no designated upper limit, however it is always more optimal to keep the amount of excess gel as low as possible to ensure that the gel slice completely dissolves prior to proceeding with the purification procedure.
Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
 |
99 / 100 Bioz Stars |
Uncleaved RNA transcripts were extracted from denaturing (8.3M ura) 10% polyacrylamide gel using the ZR small-RNA PAGE Recovery kit. This facilitates the generation of ribozyme-based control devices for gene regulatory activities.
The ZR Small-RNA PAGE Recovery kit was used to gel purify short RNAs (50-250 bp) from isolated total RNA on a 15% TBE Urea gel for downstream quantitative PCR.
In vitro transcribed short hairpin RNA constructs were recovered from 20% denaturing polyacrylamide gels using the ZR small-RNA PAGE Recovery kit. This was used to show that influenza A virus panhandle structure could bind to retinoic acid-inducible gene I (RIG-I) and affect type I interferon production.
