ZR Urine RNA Isolation Kit

ZR Urine RNA Isolation Kit

Cat # Name Size
R1038 ZR Urine RNA Isolation Kit 20 preps
R1039 ZR Urine RNA Isolation Kit 50 preps

Documents

Protocol:

SDS (MSDS):

Categories: , ,

Description

R1038 / R1039

Highlights

Quick & Easy: Recovery of total RNA from cells, biological sediment in urine, large volume liquid samples and suitable for isolation of RNA from microvesicles. Not for cell-free isolation.

 

High-Quality: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-PCR, etc.

Description

ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe (not provided) and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated Urine RNA Buffer. The collected lysate can then be used immediately or at a later time following transportation and/or storage. Also, the Urine RNA Buffer is ideal for direct isolation of RNA from microvesicles that may be recovered from urine filtrates. One-step RNA isolation occurs via matrix adsorption using Zymo-Spin™ IC Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.

Technical Specifications

Equipment

Microcentrifuge, syringes (30 ml, 1 ml)

Purity

RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.

Sample Source

Urine and other aqueous samples containing cells, biological sediment, microvesicle-associated RNA, etc.

Size Range

Total RNA ≥ 17 nt

Yield

10 µg RNA (binding capacity), ≥6 µl (elution volume)

Citation

RNA was isolated from whole urine using the ZR Urine RNA kit. Researchers found that microvesicles RNA is better preserved compared to whole cells, indicating possible RNA protection during urine passage.

Miranda, KC et al. Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney International. 2010.

The ZR Urine RNA kit was used to extract RNA from sloughed cells (5 mL urine) for downstream RT-PCR. A multiplex biomarker panel was developed for the diagnosis of transitional bladder carcinoma via a non-invasive method.

Kumar, P et al. Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma. Oncotarget. 2015.

Total RNA was extracted from freshly voided urine using the ZR Urine RNA kit for gene expression analysis. Aurora A Kinase (AURKA) mRNA was identified as a possible diagnostic and prognostic biomarker for urothelial bladder cancer.

de Martino, M et al. Aurora A Kinase as a diagnostic urinary marker for urothelial bladder cancer. World Journal of Urology. 2015.

Kit Components

Kit Components

Cat # Name Size
W1001-1 DNase/RNase-Free Water 1 ml
R1003-3-12 RNA Wash Buffer 12 ml
R1003-3-24 RNA Wash Buffer 24 ml
R1038-2-20 Urine RNA Buffer 20 ml
R1038-2-50 Urine RNA Buffer 50 ml
R1060-2-10 RNA Prep Buffer 10 ml
R1060-2-25 RNA Prep Buffer 25 ml
C1001-50 Collection Tubes 50 Pack
C1004-50 Zymo-Spin IC Columns 50 Pack
C1009-20 ZRC GF Filter 20 Pack
C1009-50 ZRC GF Filter 50 Pack

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