Zymo-Seq RiboFree Total RNA Library Kit
| Cat # | Name | Size |
| R3000 | Zymo-Seq RiboFree Total RNA Library Kit | 12 Preps |
| R3003 | Zymo-Seq RiboFree Total RNA Library Kit | 96 Preps |
Documents
Protocol: R3000/R3003
Protocol: R3000/R3003S
Data Sheet
SDS (MSDS): R3000
SDS (MSDS): R3003
Barcode Sequences Table:
Description
R3000 / R3003
Highlights
The Fastest & Easiest Total RNA-Seq Library Kit: Prepare stranded, RiboFree libraries from total RNA in 3.5 hours.
Compatible with Any Organism: Probe-free technology depletes rRNA & Globin from any RNA source.
The Most Accurate: Eliminate bias from rRNA depletion.
Description
Zymo-Seq RiboFree Total RNA-Seq Library Kit is the fastest, easiest RNA-Seq library prep kit available to make stranded, total RNA libraries. The automation-friendly protocol minimizes hands-on time to generate sequencer-ready, stranded, indexed libraries depleted of rRNA and globin in as little as 3.5 hours.
Depletion of ribosomal RNAs (which can comprise as much as 90% of a total RNA sample) is completely integrated into the workflow, using a novel, probe-free technology that removes rRNA, globin, or any other over-abundant, repetitive transcript from any sample type or species.
RiboFree Universal Depletion is validated across biological kingdom and phyla, including human, rodent, plant, and various prokaryotes, as well as RNA from a wide range of sample types including cells, tissue, FFPE tissues, and whole blood, eliminating the need to select sample-specific modules for different sample types. Just one total RNA-Seq
library prep kit to deplete them all.
Because the RiboFree Universal Depletion is probe-free, researchers using the Zymo-Seq RiboFree Total RNA Library Kit will avoid transcriptome profiling bias found in other methods. High concentrations of rRNA-binding oligonucleotides used in probe-based approaches also bind to a significant number of non-ribosomal targets. As much as 20% of the 20,000 protein-coding genes in the human genome are significantly biased by these methods.
In contrast, the probe-free depletion developed by Zymo Research uses only abundant pre-existing transcripts for mismatch-free enzymatic removal of rRNA and globin, resulting in minimal off-target effects as low as 1.8% in all protein-coding transcripts.
This total RNA-Seq library prep kit’s protocol is fragmentation-free and works with up to 2 ug of undepleted total RNA, down to 50 ng of undepleted total RNA, or as little as 5 ng of ribosome-depleted RNA samples. Pre-mixed reagents are ready-to-use at every step, eliminating the need for manual master-mix preparation. Everything needed to make sequencing-ready libraries, including indexing primers, SPRI beads, and magnetic bead stand are included in the kit. It’s RNA-Seq made simple.
Equipment Required
Thermocycler, magnet stand*, and microcentrifuge.
*for a complimentary magnet stand, please contact tech@zymoresearch.com
Input Quality
Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion
Maximum Input
5 µg
Minimum Input
100 ng
Processing Time
As little as 3.5 hours (RNA to indexed library)
Recommended Input
500 ng
Sequencing
Libraries are stranded and compatible with all Illumina® sequencing platforms. The Read 1 sequence will be antisense to the RNA molecule of origin.
Sample Input Material
RNA from any species
Q1: Do you sell the ribo depletion separately?
Yes, please contact technical support tech@zymoresearch.com for ordering information.
Q2: Will this kit work with prokaryotes?
Yes, this kit will work with any organism. As long as your sample has rRNA, this kit will work.
Q3: My sample is lower than 100 ng, will this kit still work?
Lower sample inputs will require a longer incubation time during RiboFree Universal Depletion. Please refer to Appendix B for suggestions
“This is probably the best RNA-seq library preparation kit I’ve used (and I have used many). It is very simple and gives clean, reproducible results at a very affordable price. I would highly recommend this product to anyone looking for a quicker and easier library preparation kit. Zymo has never let me down.”
– Galina (City of Hope)
“I think this kit is faster and simpler to do than the Truseq RNA library prep kit. It was easy enough that I tried it once and got a nice library prep, with a high concentration and I did not make any mistakes in my first try! I will definitely buy this kit and recommend it to others without a doubt.”
– Rafael (UC Berkeley)
“This RNA Library Kit was easy to use and recovered good efficiency.”
– Veronica L (CICY Mexico)
| Cat # | Name | Size |
|---|---|---|
| D3004-4-10 | DNA Elution Buffer | 10 ml |
| D3004-4-50 | DNA Elution Buffer | 50 ml |
| W1001-1 | DNase/RNase-Free Water | 1 ml |
| W1001-10 | DNase/RNase-Free Water | 10 ml |
| D4084-10 | Select-a-Size MagBead Set | 10 ml |
| D4084-50 | Select-a-Size MagBead Set | 50 ml |
| E2003 | ZymoTaq PreMix | (50 Rxns.) (2 x 625 µl) |
| E2004 | ZymoTaq PreMix | (200 Rxns.) (8 x 625 µl) |
| R3001 | Zymo-Seq RiboFree Universal cDNA Kit | 12 Preps |
| D3008 | Zymo-Seq UDI Primer Set (Indexes 1-12) | 12 indexes |
| D3096 | Zymo-Seq UDI Primer Plate (Indexes 1-96) | 96 Indexes |
| R3004-1-6 | Zymo-Seq Wash Buffer | 6 mL |
| R3004-1-48 | Zymo-Seq Wash Buffer | 48 mL |
| 3DP-1002 | PCR Strip MagStand | 1 unit |
