Zymo-Seq RiboFree Universal cDNA Kit

Zymo-Seq RiboFree Universal cDNA Kit

Cat # Name Size
R3001 Zymo-Seq RiboFree Universal cDNA Kit 12 Preps

Documents

Protocol

Data Sheet

SDS (MSDS)

Categories: , ,

Description

R3001

Highlights

The Easiest Kit: Prepare RiboFree cDNA from total RNA in as little as 5 pipetting steps.

Compatible with Any Sample: Probe-free technology depletes rRNA & globin from any RNA source.

The Most Accurate: Eliminate bias from rRNA depletion.

Description

The Zymo-Seq RiboFree Universal cDNA Kit produces first-strand cDNA, and is the easiest rRNA and globin depletion kit available. The RiboFree Universal Depletion is compatible with RNA from all biological sample types, tissues (including whole blood), and does not require organism-specific probes to deplete rRNA and globin. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-strand cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, and the probe-free method eliminates transcriptome profile bias compared to popular rRNA depletion and poly(A) enrichment kits.

Equipment Required

Thermocycler, magnet stand (free at checkout), and microcentrifuge

Input Quality

Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion

Maximum Input

5 µg

Minimum Input

100 ng

Processing TIme

As little as 1.5 hours (RNA to single-stranded cDNA)

Recommended Input

500 ng

Sample Input Material

RNA

Q1: Does this kit yield RNA?

No, the final product is single-stranded cDNA.

 

Q2: Is this part of a library prep kit?

Yes, this is included in the Zymo-Seq RiboFree Total RNA Library Kit

 

Q3: What library prep kit is this compatible with?

This is compatible with any library prep method that uses single-stranded DNA as an input.

 

Q4: Will this kit work with prokaryotes?

Yes, this kit will work with any organism.

 

Q5: My sample is lower than 100 ng, will this kit still work?

Lower sample inputs will require a longer incubation time during RiboFree Universal Depletion. Please refer to Appendix B for suggestions.

Cat # Name Size
D3004-4-1 DNA Elution Buffer 1 ml
W1001-1 DNase/RNase-Free Water 1 ml
D4084-10 Select-a-Size MagBead Set 10 ml
D4084-50 Select-a-Size MagBead Set 50 ml
R3004-1-6 Zymo-Seq Wash Buffer 6 mL
3DP-1002 PCR Strip MagStand 1 unit