Zymo-Seq RiboFree Universal cDNA Kit (12 preps)
| Cat# | Name | Size |
|---|---|---|
| R3001 | Zymo-Seq RiboFree Universal cDNA Kit | 12 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS):
Description
Highlights
- The Easiest Kit: Prepare RiboFree cDNA from total RNA in as little as 5 pipetting steps.
- Compatible with Any Sample: Probe-free technology depletes rRNA & globin from any RNA source.
- The Most Accurate: Eliminate bias from rRNA depletion.
Description
The Zymo-Seq RiboFree Universal cDNA Kit produces first-strand cDNA, and is the easiest rRNA and globin depletion kit available. The RiboFree Universal Depletion is compatible with RNA from all biological sample types, tissues (including whole blood), and does not require organism-specific probes to deplete rRNA and globin. The RiboFree single-stranded cDNA produced by the kit can be used for qPCR detection, RNA-Seq library prep, and any other downstream analysis. In contrast to poly(A) enrichment methods, the RiboFree Universal cDNA Kit produces single-strand cDNA from nearly all RNA biotypes, including full-length mRNA, mRNA with degraded poly(A) tails, long-noncoding RNA (lncRNA), intronic RNA, nucleolar RNA, etc. The cost-effective Zymo-Seq RiboFree Universal cDNA Kit has been optimized to reduce hands-on time, and the probe-free method eliminates transcriptome profile bias compared to popular rRNA depletion and poly(A) enrichment kits.
Equipment Required
Thermocycler, magnet stand (free at checkout), and microcentrifuge
Input Quality
Ensure RNA A260/A280 and A260/A230 ratios are ≥ 1.8, DNA-free, and PCR inhibitor-free for high-fidelity cDNA transcription and depletion
Maximum Input
5 µg
Minimum Input
100 ng
Processing Time
As little as 1.5 hours (RNA to single-stranded cDNA)
Recommended Input
500 ng
Sample Input Material
RNA
Q1: Does this kit yield RNA?
No, the final product is single-stranded cDNA.
Q2: Is this part of a library prep kit?
Yes, this is included in the Zymo-Seq RiboFree Total RNA Library Kit
Q3: What library prep kit is this compatible with?
This is compatible with any library prep method that uses single-stranded DNA as an input.
Q4: Will this kit work with prokaryotes?
Yes, this kit will work with any organism.
Q5: My sample is lower than 100 ng, will this kit still work?
Lower sample inputs will require a longer incubation time during RiboFree Universal Depletion. Please refer to Appendix B for suggestions.
“This is probably the best RNA-seq library preparation kit I’be used (and I have used many). It is very simple and gives clean, reproducible results at a very affordable price. I would highly recommend this product to anyone looking for a quicker and easier library preparation kit. Zymo has never let me down.”
– Galina (City of Hope)
“I think this kit is faster and simpler to do than the Truseq RNA library prep kit. It was easy enough that I tried it once and got a nice library prep, with a high concentration and I did not make any mistakes in my first try! I will definitely buy this kit and recommend it to others without a doubt.”
– Rafael (UC Berkeley)
“This RNA Library Kit was easy to use and recovered good efficiency.”
– Veronica L (CICY Mexico)
| Cat# | Name | Size |
|---|---|---|
| D3004-4-1 | DNA Elution Buffer | 1 ml |
| W1001-1 | DNase/RNase-Free Water | 1 ml |
| D4084-10 | Select-a-Size MagBead Set | 10 ml |
| D4084-50 | Select-a-Size MagBead Set | 50 ml |
| R3004-1-6 | Zymo-Seq Wash Buffer | 6 ml |
| 3DP-1002 | PCR Strip MagStand | 1 unit |
- Catalog#: R3001
- Package Length (in Inches): 12
- Package Width (in Inches): 9
- Package Height (in Inches): 10
- Size: 12 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
