Zymo-Seq UDI Primer Set
| Cat# | Name | Size |
|---|---|---|
| D3008 | Zymo-Seq UDI Primer Set (Indexes 1-12) | 12 indexes |
Documents
Protocol:
SDS (MSDS):
Barcode Sequences Table:
Description
Description
The Zymo-Seq UDI Primer Sets contain pre-mixed Unique Dual Index (UDI) primers that can be used to barcode any library containing TruSeq® adapters. Indexes in the Zymo-Seq UDI Primer Set (Indexes 1-12) are dispensed in 1.5 mL tubes (Cat. No. D3008), and the Zymo-Seq UDI Primer Plate (Indexes 1-96) are dispensed in single-use foil-sealed 96-well plates (Ca. No. D3096). Indexed libraries are compatible with any Illumina® sequencer. These indexes are compatible with the Zymo-Seq RiboFree Total RNA Library Kit
Q1: Are these individual primers or pre-mixed primer sets?
Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 µM total (2.5 µM each primer).
Q2: What are the sequences of the indexes?
Please view the “Zymo-Seq UDI Primer Set 1-96” spreadsheet for index sequences and adapter trimming parameters.
Q3: Will this work with Nextera adapters?
No, these index primers only work with TruSeq compatible libraries.
- Catalog#: D3008
- Package Length (in Inches): 6
- Package Width (in Inches): 4
- Package Height (in Inches): 0.5
- Size: 20 ul / index
- Unit Standard: Metric
- Volume Units: Milliliters
