Zymo-Seq UDI Primer Sets
| Cat # | Name | Size |
| D3008 | Zymo-Seq UDI Primer Set (Indexes 1-12) | 12 indexes |
| D3096 | Zymo-Seq UDI Primer Plate (Indexes 1-96) | 96 Indexes |
Documents
Protocol
SDS (MSDS)
Barcode Sequences Table
Description
Description
The Zymo-Seq UDI Primer Sets contain pre-mixed Unique Dual Index (UDI) primers that can be used to barcode any library containing TruSeq® adapters. Indexes in the Zymo-Seq UDI Primer Set (Indexes 1-12) are dispensed in 1.5 mL tubes (Cat. No. D3008), and the Zymo-Seq UDI Primer Plate (Indexes 1-96) are dispensed in single-use foil-sealed 96-well plates (Ca. No. D3096). Indexed libraries are compatible with any Illumina® sequencer. These indexes are compatible with the Zymo-Seq RiboFree Total RNA Library Kit.
Q1: Are these individual primers or pre-mixed primer sets?
Each tube contains a pre-mixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentration of each UDI primer pre-mix is 5 µM total (2.5 µM each primer).
Q2: What are the sequences of the indexes?
Please view the “Zymo-Seq UDI Primer Set 1-96” spreadsheet for index sequences and adapter trimming parameters.
Q3: Will this work with Nextera adapters?
No, these index primers only work with TruSeq compatible libraries.
