ZymoBIOMICS DNA Miniprep Kit
| Cat# | Name | Size |
|---|---|---|
| D4300 | ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) | 50 Preps |
| D4300T | ZymoBIOMICS DNA Miniprep Kit | 5 Preps |
| D4304 | ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) | 50 Preps |
Documents
Protocol:
Datasheet:
SDS (MSDS): D4300
SDS (MSDS): D4304
Optimized Lysis Protocols
Description
Highlights
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Applicable For
All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume
≥ 50 µl DNA Elution Buffer or DNase free water
Equipment
Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume
Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109 bacterial, 2×108 yeast, or 2×107 mammalian cells)
Purity
High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type
Total DNA
Yield
Up to 25 µg total DNA can be eluted into ≥50 µl
 |
98 / 100 Bioz Stars |
Metagenome sequencing-based strain-level and functional characterization…
J Oral Microbial – Published 28 Dec 2018
Sterile forceps (a new pair for every sample) were used to remove the points from the tubes.. Each plaque sample was pelleted by centrifugation at maximum speed, washed twice in 1 ml PBS, and digested by re-suspending in 250 μl PBS containing 25 μl Metapolyzme (Sigma, USA), and incubating at 37° for 3 h. The lysate was used for DNA extraction employing the ZymoBiomics miniprep kit (Zymo Research, Germany) according to the manufacturer’s instructions.. Quantity and quality of the extracts were assessed using a Qubit 3.0 (ThermoFisher Scientific, USA).
Genotype-Environment Interaction Shapes the Microbial Assemblage in Grap..
Microorganisms – Published 21 Sep 2018
DNA was extracted from each sample by using the ZymoBiomics DNA MicroPrep Kit (Zymo Research, Irvine, CA, USA) following the manufacturer’s instructions.
Assembly of 913 microbial genomes from metagenomic sequencing of the cow…
Nat Commun – Published 28 Feb 2018
Briefly, the sample was spun down for 2 min at 6000 rpm, rinsed with PBS and spun down again (5 min at 6000 rpm), prior to removing the supernatant.. Cells were lysed with glass bead disruption in a detergent buffer, and DNA was extracted using the ZymoBiomics DNA Mini Kit (Zymo Research).. For shotgun library creation, 100 ng was sheared to ~400 bp average insert length and used to create a library using the HyperPrep kit (KAPA Biosystems).
| Cat # | Name | Size |
|---|---|---|
| D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml |
| D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml |
| D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml |
| D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml |
| D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml |
| D4302-5-1 | ZymoBIOMICS DNase/RNase Free Water | 1 ml |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml |
| C1057-50 | Zymo-Spin III-F Filters | 50 Pack |
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| C1078-50 | Zymo-Spin IICR Columns | 50 Pack |
- Catalog#: D4300 /D4300T /D4304
- Package Length (in Inches): 7 /2.5 /9
- Package Width (in Inches): 5 /2.2 /6.5
- Package Height (in Inches): 4.5 /1/ 6
- Package Weight (in Pounds): 2.75 /0.35 /2.2
- Size: 50 Preps /5 Preps /50 Preps
- Unit Standard: Metric
- Volume Units: Milliliters
